IDENTIFIKASI BAKTERI ASAM LAKTAT DARI KOMBUCHA ROSELLA

Merkuria - Karyantina, Sumarmi Sumarmi

Abstract


Kombucha is one type of traditional fresh drink derived from fermented sweet tea water for 7-10 days. Bacteria play a role in the kombucha fermentation process are acetic acid acid bacteria and lactic acid bacteria. This study aims to isolation and identification lactic acid bacteria that play a role in the kombucha rosella fermentation. The study was conducted using De Man, Rogosa and Sharpe media to add CaCO3 1 %. Colonies that grow and provide clear zones are purified several times to obtain uniform colonies morphologically and biochemically. The 18 isolates identified 8 isolates that have the characteristics of lactic acid bacteria. Analysis with API 50CH KIT identified 4 isolates from the genera Lactobacillus and 4 isolates from the genus Lactococcus.


Keywords


kombucha roselle; probiotic; isolate; Lactobacillus; Lactococcus

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DOI: https://doi.org/10.21107/agrointek.v15i1.7340

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