Bintang Sipartogi Panjaitan, Linda Lestari, Radite Praeko Agus Setiawan, Armansyah Halomoan Tambunan


The sustainability of hydrogen as an energy carrier depends on the production process and the source of raw materials. The choice of substrate in anaerobic digestion process plays an important role to maximize biohydrogen production because it depends on its availability and the composition of substrate. The purpose of this study is to design and construct a prototype reactor for biohydrogen production and to determine the potential of H2 gas production from anaerobic digestion process. This study uses an experimental research method with three operating temperature variations in the reactor, at the range of thermophilic temperatures, i.e. 55°C, 60°C, and 65°C. The substrate used was POME and cow dung, and the process was conducted in 24 hours which is assumed to be the stage of non-methanogenic within the anaerobic process. From this research, the prototype of continuous stirred tank reactor (CSTR) in batch system was made from acrylic, with a capacity of 6 liters biomass waste. Using the reactor, total biohydrogen gas produced during 24 hours process with cow manure as substrate was 0,0932 gram at 55°C; 0,0307 gram at 60°C and 0,0797 gram at 65°C. While, biohydrogen production using POME as substrate was 0,0645 gram at 55°C; 0,1708 gram at 60°C, and 0,0636 gram at 65°C. These results indicate the potentiality of POME and cow manure to produce biohydrogen gas during anaerobic digestion process.


Anaerobic digestion; biohydrogen; dark fermentation; thermophilic


Assawamongkholsiri, T., Reungsang, A., 2015. Photo-fermentational hydrogen production of Rhodobacter sp. KKU-PS1 isolated from an UASB reactor. Electron. J. Biotechnol. 18, 221–230. https://doi.org/10.1016/j.ejbt.2015.03.011

Bartacek, J., Zabranska, J., Lens, P.N.L., 2007. Developments and constraints in fermentative hydrogen production. Biofuels, Bioprod. Biorefining. https://doi.org/10.1002/bbb.17

Carere, C.R., Sparling, R., Cicek, N., Levin, D.B., 2008. Third generation biofuels via direct cellulose fermentation. Int. J. Mol. Sci. https://doi.org/10.3390/ijms9071342

Chinwendu, S., Chibueze, U., Tochukwu, E., 2013. Anaerobic Digester Considerations of Animal Waste. Am. J. Biochem. 2013, 93–96. https://doi.org/10.5923/j.ajb.20130304.02

Chong, M.L., Sabaratnam, V., Shirai, Y., Hassan, M.A., 2009. Biohydrogen production from biomass and industrial wastes by dark fermentation. Int. J. Hydrogen Energy. https://doi.org/10.1016/j.ijhydene.2009.02.010

Ghimire, A., Frunzo, L., Pirozzi, F., Trably, E., Escudie, R., Lens, P.N.L., Esposito, G., 2015. A review on dark fermentative biohydrogen production from organic biomass: Process parameters and use of by-products. Appl. Energy. https://doi.org/10.1016/j.apenergy.2015.01.045

Guo, X.M., Trably, E., Latrille, E., Carrre, H., Steyer, J.P., 2010. Hydrogen production from agricultural waste by dark fermentation: A review. Int. J. Hydrogen Energy 35, 10660–10673. https://doi.org/10.1016/j.ijhydene.2010.03.008

Hallenbeck, P.C., Ghosh, D., 2009. Advances in fermentative biohydrogen production: the way forward? Trends Biotechnol. https://doi.org/10.1016/j.tibtech.2009.02.004

Jung, K.W., Kim, D.H., Kim, S.H., Shin, H.S., 2011. Bioreactor design for continuous dark fermentative hydrogen production. Bioresour. Technol. 102, 8612–8620. https://doi.org/10.1016/j.biortech.2011.03.056

Kothari, R., Singh, D.P., Tyagi, V. V., Tyagi, S.K., 2012. Fermentative hydrogen production - An alternative clean energy source. Renew. Sustain. Energy Rev. https://doi.org/10.1016/j.rser.2012.01.002

Lay, J.J., Fan, K.S., Chang I, J., Ku, C.H., 2003. Influence of chemical nature of organic wastes on their conversion to hydrogen by heat-shock digested sludge. Int. J. Hydrogen Energy 28, 1361–1367. https://doi.org/10.1016/S0360-3199(03)00027-2

Li, R., Chen, S., Li, X., 2010. Biogas production from anaerobic co-digestion of food waste with dairy manure in a two-phase digestion system, in: Applied Biochemistry and Biotechnology. pp. 643–654. https://doi.org/10.1007/s12010-009-8533-z

Monlau, F., Sambusiti, C., Ficara, E., Aboulkas, A., Barakat, A., Carrère, H., 2015. New opportunities for agricultural digestate valorization: Current situation and perspectives. Energy Environ. Sci. https://doi.org/10.1039/c5ee01633a

O-Thong, S., Prasertsan, P., Intrasungkha, N., Dhamwichukorn, S., Birkeland, N.Kå., 2008. Optimization of simultaneous thermophilic fermentative hydrogen production and COD reduction from palm oil mill effluent by Thermoanaerobacterium-rich sludge. Int. J. Hydrogen Energy 33, 1221–1231. https://doi.org/10.1016/j.ijhydene.2007.12.017

Pan, J., Zhang, R., El-Mashad, H.M., Sun, H., Ying, Y., 2008. Effect of food to microorganism ratio on biohydrogen production from food waste via anaerobic fermentation. Int. J. Hydrogen Energy 33, 6968–6975. https://doi.org/10.1016/j.ijhydene.2008.07.130

Park, M.J., Jo, J.H., Park, D., Lee, D.S., Park, J.M., 2010. Comprehensive study on a two-stage anaerobic digestion process for the sequential production of hydrogen and methane from cost-effective molasses. Renew. Energy 35, 6194–6202. https://doi.org/10.1016/j.ijhydene.2010.03.135

Sreela-Or, C., Imai, T., Plangklang, P., Reungsang, A., 2011. Optimization of key factors affecting hydrogen production from food waste by anaerobic mixed cultures, in: International Journal of Hydrogen Energy. pp. 14120–14133. https://doi.org/10.1016/j.ijhydene.2011.04.136

Sung, S., Liu, T., 2003. Ammonia inhibition on thermophilic anaerobic digestion. Chemosphere 53, 43–52. https://doi.org/10.1016/S0045-6535(03)00434-X

Zhu, H., Parker, W., Basnar, R., Proracki, A., Falletta, P., Béland, M., Seto, P., 2009. Buffer requirements for enhanced hydrogen production in acidogenic digestion of food wastes. Bioresour. Technol. 100, 5097–5102. https://doi.org/10.1016/j.biortech.2009.02.066

Zong, W., Yu, R., Zhang, P., Fan, M., Zhou, Z., 2009. Efficient hydrogen gas production from cassava and food waste by a two-step process of dark fermentation and photo-fermentation. Biomass and Bioenergy 33, 1458–1463. https://doi.org/10.1016/j.biombioe.2009.06.008





  • There are currently no refbacks.

Copyright (c) 2021 Bintang Sipartogi Panjaitan, Linda Lestari, Radite Praeko Agus Setiawan, Armansyah Halomoan Tambunan

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.