EFEKTIVITAS BERBAGAI PELARUT ORGANIK PADA EKSTRAKSI SENYAWA FUNGSIONAL BERAS HITAM

Nurhidajah Nurhidajah, Ali Rosidi, Yunan Kholifatuddin Sya'di, Diode Yonata

Abstract

Functional compounds from black rice were extracted conventionally using organic solvents, namely ethanol-distilled water and ethanol-distilled water-acid (acetic acid and a mixture of lactic acid and acetic acid) along with distilled water as control. The effectiveness of each solvent was determined by analysis of functional compounds contents (anthocyanin content, flavonoids, total phenolic, and antioxidant activity), pH value, and the resulting color characteristics. The ethanol-distilled water-citric acid solvent produced significantly higher levels of anthocyanin, total phenolic, and antioxidant activity, with the lowest extraction pH, but produced fewer flavonoids. Red-purple black rice extract has the lowest brightness. The ethanol-distilled water-citric acid solvent has the best effectiveness in the extraction of black rice functional compounds.

Keywords

Black rice; functional compunds; extraction; organic solvent;

References

Alothman, M., Bhat, R., & Karim, A. A. (2009). Antioxidant capacity and phenolic content of selected tropical fruits from Malaysia, extracted with different solvents. Food Chemistry, 115(3), 785–788. https://doi.org/10.1016/j.foodchem.2008.12.005

Cai, Z., Qu, Z., Lan, Y., Zhao, S., Ma, X., Wan, Q., Jing, P., & Li, P. (2016). Conventional, ultrasound-assisted, and accelerated-solvent extractions of anthocyanins from purple sweet potatoes. Food Chemistry, 197, 266–272. https://doi.org/10.1016/j.foodchem.2015.10.110

Caparino, O. A., Tang, J., Nindo, C. I., Sablani, S. S., Powers, J. R., & Fellman, J. K. (2012). Effect of drying methods on the physical properties and microstructures of mango (Philippine “Carabao” var.) powder. Journal of Food Engineering, 111(1), 135–148. https://doi.org/10.1016/j.jfoodeng.2012.01.010

Chaudhary, B., & Mukhopadhyay, K. (2012). Syzygium cumini (L.) skeels: a potential source of nutraceuticals. International Journal of Pharmacy and Biological Sciences, 2(1), 46–53. www.ijpbs.com

Chua, M. T., Tung, Y. T., & Chang, S. T. (2008). Antioxidant activities of ethanolic extracts from the twigs of Cinnamomum osmophloeum. Bioresource Technology, 99(6), 1918–1925. https://doi.org/10.1016/j.biortech.2007.03.020

Coutinho, I. B., Freitas, A., Maçanita, A. L., & Lima, J. C. (2015). Effect of water content on the acid-base equilibrium of cyanidin-3-glucoside. Food Chemistry, 172, 476–480. https://doi.org/10.1016/j.foodchem.2014.09.060

do Carmo Brito, B. de N., da Silva Pena, R., Santos Lopes, A., & Campos Chisté, R. (2017). Anthocyanins of jambolão (Syzygium cumini): extraction and pH-dependent color changes. Journal of Food Science, 82(10), 2286–2290. https://doi.org/10.1111/1750-3841.13847

Espinosa-Acosta, G., Ramos-Jacques, A. L., Molina, G. A., Maya-Cornejo, J., Esparza, R., Hernandez-Martinez, A. R., Sánchez-González, I., & Estevez, M. (2018). Stability analysis of anthocyanins using alcoholic extracts from black carrot (Daucus carota ssp. Sativus var. Atrorubens alef.). Molecules, 23(11). https://doi.org/10.3390/molecules23112744

Fatchiyah, F., Ratih, D., Sari, T., Safitri, A., & Cairns, J. R. (2020). Phytochemical compound and nutritional value in black rice from Java Island, Indonesia. In Systematic Reviews in Pharmacy (Vol. 11, Issue 7).

Fernandez-Aulis, F., Hernandez-Vazquez, L., Aguilar-Osorio, G., Arrieta-Baez, D., & Navarro-Ocana, A. (2019). Extraction and identification of anthocyanins in corn cob and corn husk from cacahuacintle maize. Journal of Food Science, 84(5), 954–962. https://doi.org/10.1111/1750-3841.14589

Goli, A. H., Barzegar, M., & Sahari, M. A. (2005). Antioxidant activity and total phenolic compounds of pistachio (Pistachia vera) hull extracts. Food Chemistry, 92(3), 521–525. https://doi.org/10.1016/j.foodchem.2004.08.020

Halee, A., Supavititpatana, P., Ruttarattanamongkol, K., Jittrepotch, N., Rojsuntornkitti, K., & Kongbangkerd, T. (2018). Effects of solvent types and citric acid concentrations on the extraction of antioxidants from the black rice bran of Oryza sativa L. CV. Hom Nin. Journal of Microbiology, Biotechnology and Food Sciences, 8(2), 765–769. https://doi.org/10.15414/jmbfs.2018.8.2.765-769

He, S., Lou, Q., Shi, J., Sun, H., Zhang, M., & Li, Q. (2017). Water extraction of anthocyanins from black rice and purification using membrane separation and resin adsorption. Journal of Food Processing and Preservation, 41(4). https://doi.org/10.1111/jfpp.13091

Ito, V. C., Zielinski, A. A. F., Demiate, I. M., Spoto, M., Nogueira, A., & Lacerda, L. G. (2019). Gamma radiation effects on physicochemical, microbiological and antioxidant properties of black rice (Oryza Sativa L.) flour during storage. Carpathian Journal of Food Science and Technology, 11(3), 163–174. https://doi.org/10.34302/crpjfst/2019.11.3.14

Jha, P., Das, A. J., & Deka, S. C. (2017). Optimization of ultrasound and microwave assisted extractions of polyphenols from black rice (Oryza sativa cv. Poireton) husk. Journal of Food Science and Technology, 54(12), 3847–3858. https://doi.org/10.1007/s13197-017-2832-0

Kong, K. W., Ismail, A., Tan, C. P., & Rajab, N. F. (2010). Optimization of oven drying conditions for lycopene content and lipophilic antioxidant capacity in a by-product of the pink guava puree industry using response surface methodology. LWT - Food Science and Technology, 43(5), 729–735. https://doi.org/10.1016/j.lwt.2009.10.011

Kopjar, M., Orsolic, M., & Pilizota, V. (2014). Anthocyanins, phenols, and antioxidant activity of sour cherry puree extracts and their stability during storage. International Journal of Food Properties, 17(6), 1393–1405. https://doi.org/10.1080/10942912.2012.714027

Kurtulbaş, E., Pekel, A. G., Bilgin, M., Makris, D. P., & Şahin, S. (2020). Citric acid-based deep eutectic solvent for the anthocyanin recovery from Hibiscus sabdariffa through microwave-assisted extraction. Biomass Conversion and Biorefinery. https://doi.org/10.1007/s13399-020-00606-3

Le, X. T., Huynh, M. T., Pham, T. N., Than, V. T., Toan, T. Q., Bach, L. G., & Trung, N. Q. (2019). Optimization of total anthocyanin content, stability and antioxidant evaluation of the anthocyanin extract from vietnamese carissa carandas l. Fruits. Processes, 7(7), 1–15. https://doi.org/10.3390/pr7070468

Liu, Y., Liu, Y., Tao, C., Liu, M., Pan, Y., & Lv, Z. (2018). Effect of temperature and pH on stability of anthocyanin obtained from blueberry. Journal of Food Measurement and Characterization, 12(3), 1744–1753. https://doi.org/10.1007/s11694-018-9789-1

Mylonaki, S., Kiassos, E., Makris, D. P., & Kefalas, P. (2008). Optimisation of the extraction of olive (Olea europaea) leaf phenolics using water/ethanol-based solvent systems and response surface methodology. Analytical and Bioanalytical Chemistry, 392(5), 977–985. https://doi.org/10.1007/s00216-008-2353-9

Nakagawa, K., & Maeda, H. (2017). Investigating pigment radicals in black rice using HPLC and multi-EPR. Journal of Oleo Science, 66(5), 543–547. https://doi.org/10.5650/jos.ess16245

O’sullivan, A. M., O’callaghan, Y. C., O’gr, M. N., Ha, M., Kerry, J. P., & O’brien, N. M. (2013). The effect of solv ents on the antio xidant acti vity in Caco-2 cells of Ir ish br own sea weed extr acts pr epar ed using acceler ated solv ent extr action (ASE). Journal of Functional Foods, 5, 940–948. https://doi.org/10.1016/j.jf

Pedro, A. C., Granato, D., & Rosso, N. D. (2016). Extraction of anthocyanins and polyphenols from black rice (Oryza sativa L.) by modeling and assessing their reversibility and stability. Food Chemistry, 191, 12–20. https://doi.org/10.1016/j.foodchem.2015.02.045

Sepahpour, S., Selamat, J., Manap, M. Y. A., Khatib, A., & Razis, A. F. A. (2018). Comparative analysis of chemical composition, antioxidant activity and quantitative characterization of some phenolic compounds in selected herbs and spices in different solvent extraction systems. Molecules, 23, 1–17. https://doi.org/10.3390/molecules23020402

Wartini, N. M., Wrasiati, L. P., Widnyani, I. A. A., Putra, G. P. G., & Wijaya, I. M. A. S. (2020). Production of natural dyes from black rice bran extract on solid to solvent ratio and various of pH solvent. IOP Conference Series: Earth and Environmental Science, 472(1). https://doi.org/10.1088/1755-1315/472/1/012008

Wong, W. H., Lee, W. X., Ramanan, R. N., Tee, L. H., Kong, K. W., Galanakis, C. M., Sun, J., & Prasad, K. N. (2015). Two level half factorial design for the extraction of phenolics, flavonoids and antioxidants recovery from palm kernel by-product. Industrial Crops and Products, 63, 238–248. https://doi.org/10.1016/j.indcrop.2014.09.049

Yamuangmorn, S., Dell, B., & Prom-u-thai, C. (2018). Effects of cooking on anthocyanin concentration and bioactive antioxidant capacity in glutinous and non-glutinous purple rice. Rice Science, 25(5), 270–278. https://doi.org/10.1016/j.rsci.2018.04.004

Yang, L., Cao, Y. L., Jiang, J. G., Lin, Q. S., Chen, J., & Zhu, L. (2010). Response surface optimization of ultrasound-assisted flavonoids extraction from the flower of Citrus aurantium L. var. amara Engl. Journal of Separation Science, 33(9), 1349–1355. https://doi.org/10.1002/jssc.200900776

DOI

https://doi.org/10.21107/agrointek.v16i1.12194

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