AKTIVITAS ANTIOKSIDAN EKSTRAK HIPOKOTIL Bruguiera gymnorhiza PADA PELARUT DAN FASE KEMATANGAN YANG BERBEDA

Sri Handayani

Abstract


The research objective was to determine the best treatment from the results of the antioxidant test, phenol content, tannins and the yield of crude extract of hypocotyl B. gymnorhyza in the mature phase in solvents with different levels of polarity. The sample extraction was carried out using a single maceration method with organic solvents according to the experimental treatment. Soaking the simplicia powder in a suitable solvent (1: 5) for 3 x 24 hours in a dark and closed container. During maceration, the samples were placed in a dark room and solvent replacement is is carried out every 24 hours. The filtrate is then evaporated vacuum at a temperature according to the boiling point of each solvent. Each treatment extract was then tested for its antioxidant strength (IC50), total phenol, tannin content and yield. The results showed that the best treatment was purple hypocotyl aceton extract of B. gymnorhiza, with an antioxidant power (IC50) of 3.98 ppm, total phenol 21.61 mg.GAE/100 mg extract, tannin 59.20 ppm and yield 4.10%bk simplicia.

Keywords


Bruguiera gymnorhiza; hypocotile; extract; antioxidant activity

References


Abed, S. A., Sirat, H. M., & Taher, M. (2013). Original article : ANTIMICROBIAL ACTIVITIES AND TOXICITY STUDY OF, 404–412.

Ahmad Talib, Aidin Tamrin, S. D. (2018). Study about potential fruit mangrove as a food alterternatif in the future. International Journal of Agronomy and Tropical Plants, 1(1), 1–8.

Bunyapraphatsara, N., Jutiviboonsuk, A., Sornlek, P., Therathanathorn, W., Aksornkaew, S., Fong, H. H. S., … Kosmeder, J. (2003). Pharmacological studies of plants in the mangrove forest. Thai Journal of Phytopharmacy, 10(2), 1–12.

Das, K., Tiwari, R. K. S., & Shrivastava, D. K. (2010). Techniques for evaluation of medicinal plant products as antimicrobial agent: Current methods and future trends. Journal of Medicinal Plants Research, 4(2), 104–111. https://doi.org/10.5897/JMPR09.030

Duh, P. Der. (1998). Antioxidant activity of burdock (Arctium lappa linné): Its scavenging effect on free-radical and active oxygen. JAOCS, Journal of the American Oil Chemists’ Society, 75(4), 455–461. https://doi.org/10.1007/s11746-998-0248-8

Dwiyanti, G., & Nurani K, H. (2014). Aktivitas Antioksidan Teh Rosela (Hibiscus sabsariffa) selama Penyimpanan pada Suhu Ruang. Prosiding Seminar Nasional Sains Dan Pendidikan Sains IX, Fakultas Sains Dan Matematika UKSW.

Eveline, Siregar, T., & Sanny. (2014). Studi Aktivitas Antioksidan pada Tomat ( Lycopersicon esculentum ) ... (Eveline dkk.), 22–28.

Handayani, S. (2017). Profil Gugus Fungsional Dan Masa Molekul Ekstrak Kasar Hipokotil, 11(L).

Handayani, S., Yuwono, S. S., Aulanni’am, & Suprayitno, E. (2015). Isolation and identification structure antioxidant active compounds of ethyl acetate fraction hypokotil Bruguiera gymnorhiza (L) Lamk. International Journal of ChemTech Research, 8(4), 1858–1867.

Handayani, V., Ahmad, A. R., Sudir, M., Etlingera, P., & Sm, R. M. (2014). Uji Aktivitas Antioksidan Ekstrak Metanol Bunga dan Daun Patikala (Etlingera elatior (Jack) R.M.Sm) Menggunakan Metode DPPH. Jurnal Pharmaceutical Sciences & Research, 1(2), 86–93.

Haq, M., Sani, W., Hossain, A. B. M. S., Taha, R. M., & Monneruzzaman, K. M. (2011). Total phenolic contents, antioxidant and antimicrobial activities of Bruguiera gymnorrhiza. Journal of Medicinal Plants Research, 5(17), 4112–4118.

Hernes, P. J., Benner, R., Cowie, G. L., Goi, M. A., Bergamaschi, B. A., & Hedges, J. I. (2001). Tannin diagenesis in mangrove leaves from a tropical estuary: A novel molecular approach. Geochimica et Cosmochimica Acta, 65(18), 3109–3122. https://doi.org/10.1016/S0016-7037(01)00641-X

Inggrid, M., & Santoso, H. (2014). EKSTRAKSI ANTIOKSIDAN DAN SENYAWA AKTIF DARI BUAH KIWI ( Actinidia deliciosa ). Lembaga Penelitian Dan Pengabdian Kepada Masyarakat, III(3), 43.

Isaza M., J. (2007). Taninos o polifenoles vegetales. Scientia et Technica, 1(33), 13–18. https://doi.org/10.22517/23447214.5817

Isnindar, Wahyuono, S., Setyowati, E. . (2011). Isolation and Identification of Antioxidant Compound of Persimmon Leaves (Diospyros kaki Thunb.) using DPPH (2,2 Diphenyl-1-Pikrilhidrazil) Method. Majalah Obat Tradisional, 16(3), 161–169.

Jacoeb, A. M., Suptijah, P., Departemen, Z., Hasil, T., Fakultas, P., Dan, P., … Pertanian Bogor, I. (2013). KOMPOSISI KIMIA, KOMPONEN BIOAKTIF DAN AKTIVITAS ANTIOKSIDAN BUAH LINDUR (Bruguiera gymnorrhiza) Chemical Composition, Bioactive Component and Antioxidant Activity of Large-Leafed Mangrove (Bruguiera gymnorrhiza) Fruit. Jphpi 2013, 16(1), 86–94.

Jaime Guerrero, C., Luigi Ciampi, P., Andrea Castilla, C., Fernando Medel, S., Heidi Schalchli, S., Emilio Hormazabal, U., … Miren Alberdi, L. (2010). Antioxidant capacity, anthocyanins, and total phenols of wild and cultivated berries in Chile. Chilean Journal of Agricultural Research, 70(4), 537–544. https://doi.org/10.4067/s0718-58392010000400002

Lestario, L., Suparmo, S., Raharjo, S., & Tranggono, T. (2005). Perubahan Aktivitas Antioksidan, Kadar Antosianin Dan Polifenol Pada Beberapa Tingkat Kemasakan Buah Duwet (Syzygium Cumini). Agritech: Jurnal Fakultas Teknologi Pertanian UGM. https://doi.org/10.22146/agritech.9444

Maesaroh, K., Kurnia, D., & Al Anshori, J. (2018). Perbandingan Metode Uji Aktivitas Antioksidan DPPH, FRAP dan FIC Terhadap Asam Askorbat, Asam Galat dan Kuersetin. Chimica et Natura Acta, 6(2), 93. https://doi.org/10.24198/cna.v6.n2.19049

Molyneux, P. (2004). The Use of the Stable Free Radical Diphenylpicryl-hydrazyl (DPPH) for Estimating Antioxidant Activity. Songklanakarin Journal of Science and Technology, 26(December 2003), 211–219. https://doi.org/10.1287/isre.6.2.144

Ncube, N. S., Afolayan, A. J., & Okoh, A. I. (2008). Assessment techniques of antimicrobial properties of natural compounds of plant origin: Current methods and future trends. African Journal of Biotechnology, 7(12), 1797–1806. https://doi.org/10.5897/AJB07.613

Niksolihin, S. (1987). Koleksi Buku 1987 Harborne , J . B . " Metode fitokimia : penuntun cara modern menganalisis tumbuhan / J . B . Harborne ; penerjemah Kosasih Padmawinata , Iwang, 1987.

Pujimulyani, ;, Raharjo, S; Marsono, Y; Santoso, U. (2010). Terhadap aktivitas antioksidan, kadar fenol, flavonoid, dan tanin terkondensasi kunir putih (, 30(3), 141–147.

Rifai, G., Rai Widarta, I. W., & Ayu Nocianitri, K. (2018). Pengaruh Jenis Pelarut Dan Rasio Bahan Dengan Pelarut Terhadap Kandungan Senyawa Fenolik Dan Aktivitas Antioksidan Ekstrak Biji Alpukat (Persea Americana Mill.). Jurnal Ilmu Dan Teknologi Pangan (ITEPA), 7(2), 22. https://doi.org/10.24843/itepa.2018.v07.i02.p03

Routaboul, J. M., Kerhoas, L., Debeaujon, I., Pourcel, L., Caboche, M., Einhorn, J., & Lepiniec, L. (2006). Flavonoid diversity and biosynthesis in seed of Arabidopsis thaliana. Planta, 224(1), 96–107. https://doi.org/10.1007/s00425-005-0197-5

Situmorang, R. O. P., & Barus, S. P. (2015). PAPER A14 - Mangrove Management as Source of Food Alternative by the Women Fishermen Group in Sei Nagalawan , North Sumatra , Indonesia, (October), 21–22.

Sudirman, S., Nurjanah, & Jacoeb, A. M. (2014). Proximate compositions, bioactive compounds and antioxidant activity from large-leafed mangrove (Bruguiera gymnorrhiza) fruit. International Food Research Journal, 21(6), 2387–2391.

Waksman De Torres, N., Salazar-Aranda, R., Pérez-López, L. A., López-Arroyo, J., & Alanís-Garza, B. A. (2011). Antimicrobial and antioxidant activities of plants from northeast of Mexico. Evidence-Based Complementary and Alternative Medicine, 2011. https://doi.org/10.1093/ecam/nep127

Young, T. (1995). Kandungan organik tumbuhan tinggi / Trevor Robinson ; penerjemah Kosasih Padmawinata ; penyunting Tetet Sutomo The Scapegoat / by Daphne Du Maurier ; adapted and brought within the vocabulary of new method reader 4 ( alternative edition ) by Michael West ; illustrated by Trevor Ridley 30 menit ... merencanakan proyek = 30 minutes ... to plan a project / Kandungan organik tumbuhan tinggi / Trevor Robinson ; penerjemah Kosasih Padmawinata ; penyunting Tetet Sutomo Instalasi listrik tingkat lanjut / Trevor Linsley ; Alih bahasa : Wiwit Kastawan ; Editor : Wayan Santika , Wibi Hardani Instalasi listrik dasar / Trevor Linsley ; Alih bahasa : Mirza Satriawan ; Editor : Wayan Santika , Wibi Hardani Exchanges : a global history reader volume I , to 1500 / Trevor R . Getz , Richard J . Hoffman , Jarbel Rodriguez Exchanges : A Global history reader volume II , from 1450 / Trevor R . Getz, 1–2.




DOI: https://doi.org/10.21107/agrointek.v15i3.8477

Refbacks

  • There are currently no refbacks.




Copyright (c) 2021 Sri Handayani

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

Indexed by

Crossref logo

Mailing Address:

Agrointek: Jurnal Teknologi Industri Pertanian

Department of Agroindustrial Technology, Faculty of Agriculture, University of Trunojoyo Madura
Raya Telang St. PO BOX 2, Kamal - Bangkalan - Jawa Timur
Phone: (031) 3013234, Email:agrointek@trunojoyo.ac.id

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License