Karakterisasi Awal Kimia, Mikrobiologi, dan Fisik “Eco Atsiri”, Campuran Eco Enzyme dan Hidrosol Sereh Wangi (Cymbopogon nardus (L.) Rendle)

Abstract

This preliminary study characterized the chemical, microbiological, and physical properties of “Eco Atsiri,” a mixture of citronella (Cymbopogon nardus (L.) Rendle) hydrosol, eco enzyme, and distilled water at a volume ratio of 1:1:10. One composite sample without replication was analyzed by a third-party accredited laboratory using the methods listed in the test report. The sample contained 0.86% total active substances, 0.11% total fatty acids, and 0.31% ethanol-insoluble matter, while free alkali was not detected (<0.002%). The laboratory reported negative results for Escherichia coli, Listeria spp., Salmonella spp., Shigella spp., Campylobacter spp., and Staphylococcus spp.; Clostridium spp. was reported at <10 CFU/mL. The sample had a pH of 4.87, a viscosity of 1.215 cP, and a moisture content of 99.09%, and mineral oil was not detected. Compared with SNI 2588:2017 for liquid hand soap, used here only as an analogous reference, pH, ethanol-insoluble matter, and free alkali were within the limits, whereas total active substances were far below the minimum; the mixture therefore cannot be claimed to conform to that standard. Because only one composite sample was analyzed and cleaning performance, deodorization, storage stability, exposure, irritation, and toxicity were not assessed, the results should be interpreted as preliminary product characterization rather than definitive evidence of safety or efficacy. Replicated batch testing, method verification for the product matrix, product-specific acceptance criteria, stability testing, and toxicological assessment are required before household or livestock-farm application.