Profil FTIR, kandungan fenolik, dan aktivitas penangkapan radikal bebas ekstrak daun talas beneng (Xanthosoma undipes K. Koch) dari pelarut dengan polaritas yang berbeda

Mohamad Ana Syabana, Septariawulan Kusumasari, Yonida Salsabila, Winda Nurtiana

Abstract

Beneng taro leaves (Xanthosoma undipes K. Koch) are Banten's local biodiversity, which has recently been used as a substitute for tobacco in cigarettes. The availability of this commodity is abundant along with increased the planted area. Therefore, it is necessary to evaluate other potency such as functional activities and also their phytochemical profiles which have not reported yet. One of importance factors that influencing functional and phytochemical profiles is the polarity of extraction solvent. The purpose of this study was to analyze the chemical profile and free radical scavenging activity of beneng taro leaves extracted with various polarities solvent. The leaves extraction was carried out using non-polar, semi-polar and polar solvents, namely hexane, chloroform, acetone and water. Their phytochemical profile was conducted by analyzing total phenolic content and FTIR spectrum, while functional activities by determining free radical scavenging activity. The results showed that the highest phenolic content was obtained from chloroform extract, while the free radical scavenging activity (IC50) was acquired from acetone extract with values of 11.24 mg GAE/g and 36.83 µg/ml, respectively. The FTIR spectrum of the hexane, chloroform, and acetone extract of beneng taro leaves had similar profiles, while the water extract was different.

Keywords

Free radical scavenging activity; FTIR; Polarity solvent; Phenolic; Xanthosoma undipes leaves extract;

References

Afriyanti, A., Yuliana, N.D., Regiyana, Y., Kusumaningrum, H.D. 2023. Identification of Antibacterial Components in Indonesian Minor Spices Using Chromatographic Fingerprinting and Multivariate Data Analysis. Journal of Herbs, Spices and Medicinal Plants, 29(4), 356–374. https://doi.org/10.1080/10496475.2023.2182860

Al Mamari, H. 2021. Phenolic Compounds: Classification, Chemistry, and Updated Techniques of Analysis and Synthesis. 1–22. https://doi.org/10.5772/intechopen.98958

Alam, S., Rashid, M.A., Sarker, M.M.R., Emon, N.U., Arman, M., Mohamed, I.N., Haque, M.R. 2021. Antidiarrheal, antimicrobial and antioxidant potentials of methanol extract of Colocasia gigantea Hook. f. leaves: evidenced from in vivo and in vitro studies along with computer-aided approaches. BMC Complementary Medicine and Therapies, 21(1), 1–12. https://doi.org/10.1186/s12906-021-03290-6

Arlin, B.D., Fadjar, K.H., Nunuk, H. 2019. Identification of Nutrition, Phytochemicals and Antioxidants Taro (Colocasia sp). 2–6. https://doi.org/10.4108/eai.30-7-2019.2287620

Boulebd, H., Zine, Y., Khodja, I.A., Mermer, A., Demir, A., Debache, A. 2022. Synthesis and radical scavenging activity of new phenolic hydrazone/hydrazide derivatives: Experimental and theoretical studies. Journal of Molecular Structure, 1249(September). https://doi.org/10.1016/j.molstruc.2021.131546

Budiarto, S., Rahayuningsih, Y. 2017. Potensi nilai ekonomi Talas Beneng (Xanthosoma undipes K.Koch) berdasarkan kandungan gizinya. Jurnal Kebijakan Pembangunan Daerah, 1(1), 1–12.

Caxito, M.L.C., Correia, R.R., Gomes, A.C.C., Justo, G., Coelho, M.G.P., Sakuragui, C.M., Kuster, R.M., Sabino, K.C.C. 2015. In Vitro Antileukemic Activity of Xanthosoma sagittifolium (Taioba) Leaf Extract. Evidence-Based Complementary and Alternative Medicine, 2015. https://doi.org/10.1155/2015/384267

Charlton, N.C., Mastyugin, M., Török, B., Török, M. 2023. Structural Features of Small Molecule Antioxidants and Strategic Modifications to Improve Potential Bioactivity. Molecules, 28(3). https://doi.org/10.3390/molecules28031057

Chawla, S., Nisha, R., Archana, S., Chatterjee, R., M, A.S., Vidya, M., Rajadurai, M. 2020. Antioxidant Analysis and Phytochemical Screening of Colocasia Esculenta Leaf Extract. Journal of Pharmaceutical Sciences and Research, 12(1), 129–132.

Domithesa, M.C., Putra, I.N.K., Wiadnyani, A.A.I.S. 2021. Pengaruh Jenis Pelarut Terhadap Aktivitas Antioksidan Ekstrak Daun Kejompot (Crassocephalum crepidioides) Menggunakan Metode Maserasi. Jurnal Ilmu Dan Teknologi Pangan (ITEPA), 10(1), 67. https://doi.org/10.24843/itepa.2021.v10.i01.p07

Correia Da Silva, T.B., Souza, V.K.T., Da Silva, A.P.F., Lyra Lemos, R.P., Conserva, L.M. 2010. Determination of the phenolic content and antioxidant potential of crude extracts and isolated compounds from leaves of Cordia multispicata and Tournefortia bicolor. Pharmaceutical Biology, 48(1), 63–69. https://doi.org/10.3109/13880200903046146

De Souza, R.F.V., Sussuchi, E.M., De Giovani, W.F. 2003. Synthesis, electrochemical, spectral, and antioxidant properties of complexes of flavonoids with metal ions. Synthesis and Reactivity in Inorganic and Metal-Organic Chemistry, 33(7), 1125–1144. https://doi.org/10.1081/SIM-120023482

Dos Santos, O.V., da Cunha, N.S.R., Duarte, S.de P.de A., Soares, S.D., da Costa, R.S., Mendes, P.M., Martins, M.G., Do Nascimento, F.D.C.A., Figueira, M.de S., Duarte, S.de P.de A., Teixeira-Costa, B.E. 2022. Determination of bioactive compounds obtained by the green extraction of taioba leaves (Xanthosoma taioba) on hydrothermal processing. Food Science and Technology (Brazil), 42, 1–7. https://doi.org/10.1590/fst.22422

Eugenio, M.H.A., Pereira, R.G.F.A., Abreu, W.C.de., Pereira, M.C.de A. 2017. Phenolic compounds and antioxidant activity of tuberous root leaves. International Journal of Food Properties, 20(12), 2966–2973. https://doi.org/10.1080/10942912.2016.1263654

Fatmawaty, A.A., Hermita, N., Hastuti, D., Kartina, A.M., Hilal, S. 2019. Phytochemical analysis of beneng taro (Xanthosoma undipes K.Koch) leaves: Cultivation as raw material for biopesticides for eco-friendly agriculture. IOP Conference Series: Earth and Environmental Science, 383(1). https://doi.org/10.1088/1755-1315/383/1/012006

Gad, H.A., Bouzabata, A. 2017. Application of chemometrics in quality control of Turmeric (Curcuma longa) based on Ultra-violet, Fourier transform-infrared and 1H NMR spectroscopy. In Food Chemistry (Vol. 237). https://doi.org/10.1016/j.foodchem.2017.06.022

Galanakis, C.M., Goulas, V., Tsakona, S., Manganaris, G.A., Gekas, V. 2013. A knowledge base for the recovery of natural phenols with different solvents. International Journal of Food Properties, 16(2), 382–396. https://doi.org/10.1080/10942912.2010.522750

Gan, C.H., Nurul A.N.B., Asmah, R. 2013. Antioxidant analysis of different types of edible mushrooms (agaricus bisporous and agaricus brasiliensis). International Food Research Journal, 20(3), 1095–1102.

Gebicki, J.M., Nauser, T. 2021. Fast antioxidant reaction of polyphenols and their metabolites. Antioxidants, 10(8). https://doi.org/10.3390/antiox10081297

Grigonis, D., Venskutonis, P.R., Sivik, B., Sandahl, M., Eskilsson, C.S. 2005. Comparison of different extraction techniques for isolation of antioxidants from sweet grass (Hierochloë odorata). Journal of Supercritical Fluids, 33(3), 223–233. https://doi.org/10.1016/j.supflu.2004.08.006

Haliza W., Kailaku, Yuliani., S. 2012. Penggunaan Mixture Response Surface Methodology Pada Optimasi Formula Brownies Berbasis Tepung Talas Banten (Xanthosama undipes K. Koch) Sebagai Alternatif Sumber Serat. J. Pascapanen, 9(2), 96–106.

Imran, M., Rasool, N., Rizwan, K., Zubair, M., Riaz, M., Zia-Ul-Haq, M., Rana, U.A., Nafady, A., Jaafar, H.Z.E. 2014. Chemical composition and Biological studies of Ficus benjamina. Chemistry Central Journal, 8(1), 1–10. https://doi.org/10.1186/1752-153X-8-12

Islamiyati, R., Pujiastuti, E. 2020. Perbandingan Uji Aktivitas Antioksidan Fraksi N-Heksan, Etil Asetat dan Air Ekstrak Etanol Kulit Buah Salak Menggunakan Metode Peredaman Radikal Bebas DPPH. Cendekia Journal of Pharmacy, 4(2), 169–174. https://doi.org/10.31596/cjp.v4i2.110

Kaczorová, D., Karalija, E., Dahija, S., Bešta-Gajević, R., Parić, A., Ćavar Zeljković, S. 2021. Influence of extraction solvent on the phenolic profile and bioactivity of two achillea species. Molecules, 26(6), 1–15. https://doi.org/10.3390/molecules26061601

Kaensombath, L., Lindberg, J.E. 2012. Effect of replacing soybean protein by taro leaf (Colocasia esculenta (L.) schott) protein on growth performance of exotic (Landrace × Yorkshire) and native (Moo Lath) lao pigs. Tropical Animal Health and Production, 45(1), 45–51. https://doi.org/10.1007/s11250-012-0172-7

Li, H.M., Hwang, S.H., Kang, B.G., Hong, J.S., Lim, S.S. 2014. Inhibitory effects of Colocasia esculenta (L.) Schott constituents on aldose reductase. Molecules, 19(9), 13212–13224. https://doi.org/10.3390/molecules190913212

Lobo, V., Patil, A., Phatak, A., Chandra, N. 2010. Free radicals, antioxidants and functional foods: Impact on human health. Pharmacognosy Reviews, 4(8), 118–126. https://doi.org/10.4103/0973-7847.70902

Marino, A., Battaglini, M., Moles, N., Ciofani, G. 2022. Natural Antioxidant Compounds as Potential Pharmaceutical Tools against Neurodegenerative Diseases. ACS Omega, 7(30), 25974–25990. https://doi.org/10.1021/acsomega.2c03291

Mofor E.G., Boudjeko, T. 2019. Different Flavonoid Profiles in Xanthosoma sagittifolium L. Schott Leaves (White and Red CV) During Growth under the Influence of Poultry Manure and NPK Fertilizers Antitumor activities of some african herbal medicine View project. Ijsrm, 13(3). www.ijsrm.humanjournals.com

Mulyati, S., Nuraeni. 2017. Studi preferensi konsumen terhadap atribut keripik talas beneng. Jurnal Agribisnis Terpadu, 9(1), 1–11.

Nandiyanto, A.B.D., Oktiani, R., Ragadhita, R. 2019. How to read and interpret ftir spectroscope of organic material. Indonesian Journal of Science and Technology, 4(1), 97–118. https://doi.org/10.17509/ijost.v4i1.15806

Nur-Hadirah, K., Arifullah, M., Nazahatul, A.A., Klaiklay, S., Chumkaew, P., Norhazlini, M.Z., Zulhazman, H. 2021. Total phenolic content and antioxidant activity of an edible Aroid, Colocasia esculenta (L.) Schott. IOP Conference Series: Earth and Environmental Science, 756(1). https://doi.org/10.1088/1755-1315/756/1/012044

Nur-Izzati, M., Arifullah, M., Nazahatul, A.A., Klaiklay, S., Chumkaew, P., Norhazlini, M.Z., Abdulhafiz, F., Zulhazman, H. 2021. Elucidation of total phenolic content and antioxidant activity in medicinal Aroid, Alocasia longiloba Miq. IOP Conference Series: Earth and Environmental Science, 756(1). https://doi.org/10.1088/1755-1315/756/1/012043

Phongpaichit, S., Nikom, J., Rungjindamai, N., Sakayaroj, J., Hutadilok-Towatana, N., Rukachaisirikul, V., Kirtikara, K. 2007. Biological activities of extracts from endophytic fungi isolated from Garcinia plants. FEMS Immunology and Medical Microbiology, 51(3), 517–525. https://doi.org/10.1111/j.1574-695X.2007.00331.x

Picerno, P., Mencherini, T., Lauro, M.R., Barbato, F., Aquino, R. 2003. Phenolic Constituents and Antioxidant Properties of Xanthosoma violaceum Leaves. Journal of Agricultural and Food Chemistry, 51(22), 6423–6428. https://doi.org/10.1021/jf030284h

Platzer, M., Kiese, S., Tybussek, T., Herfellner, T., Schneider, F., Schweiggert-Weisz, U., Eisner, P. 2022. Radical Scavenging Mechanisms of Phenolic Compounds: A Quantitative Structure-Property Relationship (QSPR) Study. Frontiers in Nutrition, 9(April), 4–8. https://doi.org/10.3389/fnut.2022.882458

Putri, N.A., Riyanto, R.A., Budijanto, S., Raharja, S. 2021. Quality Improvement of Talas Beneng ( Xanthosoma undipes K . Koch ) Flour as Banten Potential Local Product : A Preliminary Study. Journal of Tropical AgriFood, 3(2), 1–10.

Rafi, M., Meitary, N., Septaningsih, D.A., Bintang, M. 2020. Phytochemical profile and antioxidant activity of guazuma ulmifolia leaves extracts using different solvent extraction. Indonesian Journal of Pharmacy, 31(3), 171–180. https://doi.org/10.22146/ijp.598

Rohman, A., Ikhtiarini, A.N., Setyaningsih, W., Rafi, M., Aminah, N.S., Insanu, M., Irnawati, I., Santosa, D. 2021. The Use of Chemometrics for Classification of Sidaguri (Sida rhombifolia) Based on FTIR Spectra and Antiradical Activities. Indonesian Journal of Chemistry, 21(6), 1568–1576. https://doi.org/10.22146/IJC.64360

Sadek, P.C. 2002. The HPLC Solvent guide Second Edition. John Wiley and Sons.

Seal, T. 2016. HPLC determination of phenolic acids, flavonoids and ascorbic acid in four different solvent extracts of zanthoxylum acanthopodium, a wild edible plant of meghalaya state of india. International Journal of Pharmacy and Pharmaceutical Sciences, 8(3), 103–109.

Suhaendah, E., Fauziyah, E., P, L.A.G., Sudomo, A. 2021. Pertumbuhan talas beneng (Xanthosoma undipes K. Koch) pada Pola Agrofestrapi. Jurnal Agroforestri Indonesia, 4(1), 61–68.

Syabana, M.A., Yuliana, N.D., Batubara, I., Fardiaz, D. 2021. Antidiabetic activity screening and nmr profile of vegetable and spices commonly consumed in Indonesia. Food Science and Technology (Brazil), 41, 254–264. https://doi.org/10.1590/fst.14120

Umar, A.H., Ratnadewi, D., Rafi, M., Sulistyaningsih, Y.C. 2021. Untargeted metabolomics analysis using ftir and uhplc-q-orbitrap hrms of two curculigo species and evaluation of their antioxidant and α-glucosidase inhibitory activities. Metabolites, 11(1), 1–17. https://doi.org/10.3390/metabo11010042

Wakeel, A., Jan, S. A., Ullah, I., Shinwari, Z.K., Xu, M. 2019. Solvent polarity mediates phytochemical yield and antioxidant capacity of Isatis tinctoria. PeerJ, 2019(10), 1–19. https://doi.org/10.7717/peerj.7857

Wang, C.Y., Chen, Y.W., Hou, C.Y. 2019. Antioxidant and antibacterial activity of seven predominant terpenoids. International Journal of Food Properties, 22(1), 230–238. https://doi.org/10.1080/10942912.2019.1582541

Wang, X., He, X., Wang, X. 2023. FTIR Analysis of the Functional Group Composition of Coal Tar Residue Extracts and Extractive Residues. Applied Sciences (Switzerland), 13(8). https://doi.org/10.3390/app13085162

Yeshi, K., Crayn, D., Ritmejeryte, E., Wangchuk, P. 2022. Plant secondary metabolites produced in response to abiotic product development. Molecules, 27(1), 313.

Zhang, Q.W., Lin, L.G., Ye, W.C. 2018. Techniques for extraction and isolation of natural products: A comprehensive review. Chinese Medicine (United Kingdom), 13(1), 1–26. https://doi.org/10.1186/s13020-018-0177-x

DOI

https://doi.org/10.21107/agrointek.v18i4.22376

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