Physicochemical properties, antioxidant and antibacterial activities of honey produced by Heterotrigona itama and Tetragonula laeviceps

Dewi Cakrawati, Zulfikar Fadhil, Trisya Nur Adzni Destianti

Abstract

Honey is a popular healthy food that also serves as medicine. Stingless bee honey gained interest compared to regular honey due to its health benefits. This study aimed to investigate the potential health benefits of honey produced by stingless bees Heterotrigona itama and Tetragonula laeviceps, namely phenolic content, flavonoid content, antioxidant capacity, and antimicrobial activity. The research was conducted using complete randomized experimental design by two separate replications. The result shows both honeys have a pH value ranging from 3.16 to 3.43. The antioxidant capacity, total phenolic compounds, and total flavonoid compounds of both honeys were not different (p≥ 0.05). The antioxidant capacity of honey produced by Heterotrigona itama and Tetragonula laeviceps was 593 mg TE/100 and 581.58 mg TE/100 g, respectively. The total phenolic compound was in the range of 839.26-1242 mg GAE/kg, while total flavonoid was in the range 126 – 179 mg QE/ of) However, H.itama honey had the highest inhibition against Gram-positive Streptococcus mutans, while T.laeviceps had the highest inhibition against Gram-negative Pseudomonas aeruginosa. The types of local vegetation might relate to phytochemical compounds in honey. This study gave insight into another possible antibacterial compound in stingless bee honey, namely, a bioactive peptide that might act differently on microorganisms.

Keywords

Antibacterial; Antioxidant; Honey; Heterotrigona itama; Tetragonula laeviceps

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References

Amin, F.A.Z, Sabri, S., Mohammad, S.M., Ismail, M., Chan, K.W., Ismail, N., Norhaizan, M.E. and Zawawi, N. 2018. Therapeutic properties of stingless bee honey in comparison with european bee honey. Advances in Pharmacological and Pharmaceutical Sciences, 2018, Article ID 6179596

Attanzio, A., Tesoriere, L., Allegra, M. and Livrea, M. A. 2016. Monofloral honeys by Sicilian black honeybee (Apis mellifera ssp. sicula) have high reducing power and antioxidant capacity. Heliyon, 2(11), e00193. https://doi.org/10.1016/j.heliyon.2016.e00193

Ávila, S., Beux, M.R., Ribani, R.H. and Zambiazi, R.C., 2018. Stingless bee honey: Quality parameters, bioactive compounds, health-promotion properties and modification detection strategies. Trends in Food Science & Technology, 81, pp.37-50.

Boorn, K.L., Khor, Y.Y., Sweetman, E., Tan, F., Heard, T.A. and Hammer, K.A., 2010. Antimicrobial activity of honey from the stingless bee Trigona carbonaria determined by agar diffusion, agar dilution, broth microdilution and time‐kill methodology. Journal of applied microbiology, 108(5), pp.1534-1543.

Bueno-Costa, F.M., Zambiazi, R.C., Bohmer, B.W., Chaves, F.C., da Silva, W.P., Zanusso, J.T. and Dutra, I., 2016. Antibacterial and antioxidant activity of honeys from the state of Rio Grande do Sul, Brazil. LWT-Food Science and Technology, 65, pp.333-340.

Cheng, M.Z.S.Z., Ismail, M., Chan, K.W., Ooi, D.J., Ismail, N., Zawawi, N. and Mohd Esa, N., 2019. Comparison of sugar content, mineral elements and antioxidant properties of Heterotrigona itama honey from suburban and forest in Malaysia. Malays. Journal of Medical Health Science, 15, pp.104-112.

Chuttong, B., Chanbang, Y., Sringarm, K., and Burgett, M. 2016. Physicochemical profiles of stingless bee (Apidae: Meliponini) honey from South east Asia (Thailand). Food chemistry, 192, 149-155.

Deglovic, J., Majtanova, N., Majtan, J. 2022. Antibacterial and Antibiofilm Effect of Honey in the Prevention of Dental Caries: A Recent Perspective. Foods, 11, 2670. https://doi.org/10.3390/foods11172670

Dewanto, V., Wu, X., Adom, K. K. and Liu, R. H. 2002. Thermal processing enhances the nutritional value of tomatoes by increasing total antioxidant activity. Journal of agricultural and food chemistry, 50, 3010-3014.

Erban, T., Shcherbachenko, E., Talacko, P. and Harant, K., 2019. The unique protein composition of honey revealed by comprehensive proteomic analysis: Allergens, venom-like proteins, antibacterial properties, royal jelly proteins, serine proteases, and their inhibitors. Journal of natural products, 82(5), pp.1217-1226.

Hasali, N. H., Zamri, A. I., Lani, M. N., Mubarak, A., Ahmad, F., and Chilek, T. Z. T. 2018. Physico-chemical analysis and antibacterial activity of raw honey of stingless bee farmed in coastal areas in Kelantan and Terengganu. Malaysian Applied Biology, 47(4), 145-151.

Jantakee, K. and Tragoolpua, Y. 2015. Activities of different types of Thai honey on pathogenic bacteria causing skin diseases, tyrosinase enzyme and generating free radicals. Biological research, 48, 1-11. doi: 10.1186/0717-6287-48-4. PMID: 25654191; PMCID: PMC4417269

Kaškoniene, V., and Venskutonis, P. R. 2010. Floral Markers in Honey of Various Botanical and Geographic Origins: A Review. Comprehensive Reviews in Food Science and Food Safety, 9(6), 620–634. https://doi.org/10.1111/j.1541-4337.2010.00130.x

Leyva-Jimenez, F.J., Lozano-Sanchez, J., Borras-Linares, I., Cadiz- Gurrea, M.L. and Mahmoodi-Khaledi, E. (2019). Potential antimicrobial activity of honey phenolic compounds against gram positive and gram negative bacteria. LWT – Food Science and Technology, 101, 236–245.

Mahani, M., Savitri, S. R., and Subroto, E. 2022. Hubungan kadar flavonoid dan aktivitas antioksidan madu dari berbagai provinsi di Indonesia (Relation between flavonoid content and antioxidant capacity of honey from several province in Indonesia). Jurnal Sains dan Teknologi Pangan, 7(4), 5255-5268.

Nafea, E. A., Moselhy, W. A., and Fawzy, A. 2011. Does the HMF value affect the Antibacterial activity of the Bee Honey?. Egyptian academic journal of biological sciences. A, Entomology, 4(1), 13-19.

Nasir, N.M., Mohd, K.S., Ibrahim, N., Rodi, M.M.M. and Zakaria, A.J., 2019. Physicochemical analysis and antioxidant activity of honey from three Malaysian stingless bees species. Journal of Agrobiotechnology, 10(2), pp.1-11.

Nishio, E.K., Ribeiro, J.M., Oliveira, A.G., Andrade, C.G.T.J., Proni, E.A., Kobayashi, R.K.T. and Nakazato, G., 2016. Antibacterial synergic effect of honey from two stingless bees: Scaptotrigona bipunctata Lepeletier, 1836, and S. postica Latreille, 1807. Scientific reports, 6(1), p.21641.

Priawandiputra, W.. 2020. Daftar Spesies Lebah Tanpa Sengat (Stingless Bees) dan Tumbuhan Pakannya di Lubuk Bintialo dan Pangkalan Bulian, Sumatera Selatan. (List of Stingless bees and their plant feeds in Lubuk Bintialo and Pangkalan Bulian, South Sumatera). 1st edition. A.Fikty (Ed), , Zoological Society of London (ZSL) Indonesia.

Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M. and Rice-Evans, C. 1999. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free radical biology and medicine, 26, 1231-1237.

Rao, P.V., Krishnan, K.T., Salleh, N. and Gan, S.H., 2016. Biological and therapeutic effects of honey produced by honey bees and stingless bees: a comparative review. Revista Brasileira de Farmacognosia, 26, pp.657-664.

Rosli, F.N., Hazemi, M.H.F., Akbar, M.A., Basir, S., Kassim, H. and Bunawan, H., 2020. Stingless bee honey: Evaluating its antibacterial activity and bacterial diversity. Insects, 11(8),

Rozman, A.S., Hashim, N., Maringgal, B. and Abdan, K., 2022. A comprehensive review of stingless bee products: phytochemical composition and beneficial properties of honey, propolis, and pollen. Applied Sciences, 12, pp. 1-27

Sahlan, M., Mahira, K.F., Wiratama, I., Mahadewi, A.G., Yohda, M., Hermansyah, H. and Noguchi, K., 2019. Purification and characterization of proteins in multifloral honey from kelulut bee (stingless bee). Heliyon, 5(11).

Sanpa, S., Popova, M., Bankova, V., Tunkasiri, T., Eitssayeam, S. and Chantawannakul, P., 2015. Antibacterial compounds from propolis of Tetragonula laeviceps and Tetrigona melanoleuca (Hymenoptera: Apidae) from Thailand. PLoS One, 10(5), p.e0126886.doi:10.1371/journal.pone.0126886

Sulastri, E., Zubair, M.S., Anas, N.I., Abidin, S., Hardani, R. and Yulianti, R., 2018. Total phenolic, total flavonoid, quercetin content and antioxidant activity of standardized extract of Moringa oleifera leaf from regions with different elevation. Pharmacognosy journal, 10(6s). Pp. s104-s108

Suntiparapop, K., Prapaipong, P. and Chantawannakul, P., 2012. Chemical and biological properties of honey from Thai stingless bee (Tetragonula leaviceps). Journal of Apicultural Research, 51(1), pp.45-52.

Syafrizal, Ramadhan, R., Kusuma, I. W., Egra, S., Shimizu, K., Kanzaki, M. and Tangkearung, E. 2020. Diversity and honey properties of stingless bees from meliponiculture in East and North Kalimantan, Indonesia. Biodiversitas Journal of Biological Diversity, 21(10).

Tuksitha, L., Chen, Y. L. S., Chen, Y. L., Wong, K. Y., and Peng, C. C. 2018. Antioxidant and antibacterial capacity of stingless bee honey from Borneo (Sarawak). Journal of Asia-Pacific Entomology, 21(2), 563-570.

Wu, J., Han, B., Chen, X., Gao, J., Zhao, S., Sun, L. and Wang, S., 2023. Quantification of bioactive components and evaluation of microbial community and antibacterial activity from Heterotrigona itama and Tetrigona binghami honeys. International Journal of Food Science & Technology, 58(5), pp.2247-2257.

Zulhendri, F., Perera, C.O., Chandrasekaran, K., Ghosh, A., Tandean, S., Abdulah, R., Herman, H. and Lesmana, R., 2022. Propolis of stingless bees for the development of novel functional food and nutraceutical ingredients: A systematic scoping review of the experimental evidence. Journal of Functional Foods, 88, p.104902.

DOI

https://doi.org/10.21107/agrointek.v19i2.22290

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