Effect of chitosan-tripolyphosphate to suppress anthracnose (Colletotrichum spp.) in post-harvest chili

Yadi Suryadi; Dwi Ningsih Susilowati; Jajang Kosasih; Titi Tentrem; I Made Samudra

doi:10.21107/agrointek.v18i2.14368

Effect of chitosan-tripolyphosphate to suppress anthracnose (Colletotrichum spp.) in post-harvest chili

Yadi Suryadi, Dwi Ningsih Susilowati, Jajang Kosasih, Titi Tentrem, I Made Samudra

Abstract

Anthracnose disease (Colletotrichum spp.) in chili can lead to low fruit quantity and quality from planting to the postharvest stage. The environmentally friendly management using chitosan (CS) is expected to suppress anthracnose disease and increase the shelf-life of chili. This study aimed to determine the effect of the chitosan-tripolyphosphate (CS-TPP) to suppress anthracnose on chili during postharvest storage through in-vitro and in-vivo assays. In the in-vitro assay, CS-TPP solution with a ratio of [5:2] and [3:1] was applied into a warm Potato Dextrose Agar (PDA) medium. It was grown by Colletotrichum pathogen, while on in vivo assay, CS-TPP was applied to chili fruit before being inoculated by the pathogen. A control treatment was prepared without CS-TPP application. Results revealed that the CS-TPP ratios affected the growth of Colletotrichum spp. at the in-vitro assay. The CS-TPP [5:2] ratio was more effective than CS-TPP [3:1] in reducing the growth of Colletotrichum spp. with the fungal inhibition of 62,65% and 55,56%, respectively, compared to the control treatment. Moreover, it also showed anthracnose disease suppression on chili fruit of 51%, and 29%, respectively, compared to control treatment at in-vivo assay. This study showed the potential use of CS-TPP as a coating application for anthracnose disease management on storage chili, however further study such as viability and longevity of formula need to be done.

Keywords

Anthracnose; chitosan; chili; Colletotrichum spp; postharvest

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References

Al Eryani-Raqeeb, A., T.M.M. Mahmud, S.R. Syed Omar, A.R. Mohamed Zaki, and A.R. Al Eryani. 2009. Effects of calcium and chitosan treatments on controlling anthracnose and postharvest quality of papaya (Carica papaya L.). International Journal of Agricultural Research 4(2): 53-68.

Alam, M., I. Hamim, M. Ali, and M. Ashrafuzzaman. 2015. Effect of seed treatment on seedling health of chili. Journal of Environmental Science and Natural Research 7(1): 177–181.

Ali, A., N. Zahid, S. Manickam, and Y. Siddiqui. 2013. Effectiveness of submicron chitosan dispersions in controlling anthracnose and maintaining quality of dragon fruit. Postharvest Biology and Technology 86: 147–153.

Azura, M.S., I. Zamri, M.R. Rashid, and G. Shahrin. 2017. Evaluation of nanoparticles for promoting seed germination and growth rate in MR263 and MR269 paddy seeds. Journal of Tropical Agriculture and Food Science 45(1): 13–24.

BPS. 2018. Statistik sayuran 2017. BPS 2018, Jakarta, Indonesia.

Chattopadhyay, D.P., and M.S. Inamdar. 2012. Studies on synthesis, characterization and viscosity behaviour of nano chitosan. Research Journal of Engineering Sciences 1(4): 9–15.

Chookhongkha, N., T. Sopondilok, and S. Photchanachai. 2013. Effect of chitosan and chitosan nanoparticles on fungal growth and chilli seed quality. Acta Horticulture 973: 231–238.

Correa-Pacheco, Z.N., S. Bautista-Baños, M.Á. Valle-Marquina, and M. Hernández-López. 2017. The effect of nanostructured chitosan and chitosan-thyme essential oil coatings on Colletotrichum gloeosporioides growth in vitro and on cv. ‘Hass’ avocado and fruit quality. Journal of Phytopathology 165(5): 297–305. https://doi.org/10.

Dev, U., J., Akhtar, R., Chaudhury, A., Kandan, D., Chand, and J. Kumar, B. Singh, S.K. Malik, and P.C. Agarwal. 2012. Survival of Coletotrichum capsici (Syd.) Butler & Bisby in decade-long cryopreserved chilli seeds. Seed Research 40(1):92-94.

Diao, Y.Z., C. Zhang, F. Liu, W.Z. Wang, L. Liu, L. Cai, and X.L. Liu. 2017. Colletotrichum species causing anthracnose disease of chili in China. Persoonia: Molecular Phylogeny and Evolution of Fungi 38: 20–37.

Fadhil, L., A. Kadim, and A.M. Aljebory. 2014. Production of chitinase by Serratia marcescens from soil and its antifungal activity. Journal of Natural Sciences Research 4(8): 80–86.

Feliziani, E., L. Landi, and G. Romanazzi. 2015. Preharvest treatments with chitosan and other alternatives to conventional fungicides to control postharvest decay of strawberry. Carbohydrate Polymers 132: 111–117.

Flaishman, M.A., and P.E. Kolattukudy. 1994. 1994. Timing of fungal invasion using host's ripening hormone as a signal. Proceeding of National Academy Sciences USA 91 (14): 6579-6583.

Gomes, L.P., V.M.F. Paschoalin, and E.M. Del Aguila. 2017. Chitosan nanoparticles: Production, physicochemical characteristics and nutraceutical applications. Revista Virtual de Quimica 9(1): 387–409.

Hamdayanti, R. Yunita, N.N. Amin, dan T.A. Damayanti. (2017). Pemanfaatan kitosan untuk mengendalikan antraknosa pada pepaya (Colletotrichum gloeosporioides) dan meningkatkan daya simpan buah. Jurnal Fitopatologi Indonesia 8(4): 97–102. https://doi.org/10.14692/jfi.8.4.97.

Handayani, L., F. Syahputra, and Y. Astuti. 2018. Utilization and characterization of oyster shell as chitosan and nanochitosan. Jurnal Kimia Sains dan Aplikasi 21(4): 224–231.

Hassan, O., and T. Chang. 2017. Chitosan for eco-friendly control of plant disease. Asian Journal of Plant Pathology 11(2): 53–70.

James, W.C. 1971. An illustrated series of assessment keys for plant diseases. Canadian Plant Disease Survey 51(2): 39–65.

Kasaai, M.R. 2007. Calculation of Mark–Houwink–Sakurada (MHS) equation viscometric constants for chitosan in any solvent–temperature system using experimental reported viscometric constants data. Carbohydrate Polymers 3: 477-488.

Katas, H., and H.O. Alpar. 2006. Development and characterisation of chitosan nanoparticles for siRNA delivery. Journal of Controlled Release 115(2): 216–225.

Kidibule, P.E., P.S. Moriano, E.J. Ortega, M.R. Escudero, M.C. Limón, M. Remacha, F.J. Plou, J.S. Aparicio, and M.F. Lobato. 2018. Use of chitin and chitosan to produce new chitooligosaccharides by chitinase Chit42: enzymatic activity and structural basis of protein specificity. Microbial Cell Factories 17(47): 1-13.

Kim, K., and H. Ji. 2001. Effect of chitin sources on production of chitinase and chitosanase by Streptomyces griseus HUT 6037. Biotechnology and Bioprocess Engineering 6: 18-24.

Kumar, V., P.K. Gupta, V.K. Pawar, A. Verma, R. Khatik, P. Tripathi, P.P. Shukla, B. Yadav, J. Parmar, R. Dixit, P.R. Mishra, and A.K. Dwivedi. 2014. In-vitro and in-vivo studies on novel chitosan-g-pluronic f-127 copolymer based nanocarrier of amphotericin b for improved antifungal activity. Journal of Biomaterials and Tissue Engineering 4(3): 210–216.

Mahdizadeh, V., N. Safaie, and F. Khelghatibana. 2015. Evaluation of antifungal activity of silver nanoparticles against some phytopathogenic fungi and Trichoderma harzianum. Journal of Crop Protection 4: 291–300.

Mardliyati, E., S.E. Muttaqien, dan D.R. Setyawati. 2012. Sintesis nanopartikel kitosan-tripolyphosphate dengan metode gelasi ionik: pengaruh konsentrasi dan rasio volume terhadap karakteristik partikel. Prosiding Pertemuan Ilmiah Ilmu Pengetahuan Dan Teknologi Bahan 90–93.

Mulyaningtyas, D., S. Purwantisari, E. Kusdiyantini, dan Y. Suryadi. 2016. Produksi kitosan secara enzimatik oleh Bacillus firmus E65 untuk pengendalian penyakit antraknosa pada buah mangga (Mangifera indica L.). Jurnal Akademika Biologi 5(4): 8-17.

Nadia, L.M.H., P. Suptijah, and B. Ibrahim. 2014. Production and characterization chitosan nano from black tiger shrimp with ionic gelation methods. Jurnal Pengolahan Hasil Perikanan Indonesia 17(2): 119–126.

Pamekas, T., C. Sumardiyono, N. Pusposendjojo, and D. Indradewa. 2009. Extraction, characterization and inhibition test of natural chitosan to Colletotrichum musae in vitro. Jurnal Perlindungan Tanaman Indonesia 15(1): 39–44.

Pratiwi, S.U.T., E. L. Lagendijk, T. Hertiani, S. De Weert, A.M. Cornellius, and J.J. Van Den Hondel. 2015. Antimicrobial effects of Indonesian medicinal plants extracts on planktonic and biofilm growth of Pseudomonas aeruginosa and Staphylococcus aureus. International Journal of Pharmacy and Pharmaceutical Science 7(4): 183–191.

Riad, S.R., El-Mohamedy., M.M. Abdel-Kader F. Abd-El-Kareem, and N.S. El- Mougy. 2013. Inhibitory effect of antagonistic bio-agents and chitosan on the growth of tomato root rot pathogens in vitro. Journal of Agricultural Technology 9(6):1521-1533

Romanazzi, G., E. Feliziani, M. Santini, and L. Landi. 2013. Effectiveness of postharvest treatment with chitosan and other resistance inducers in the control of storage decay of strawberry. Postharvest Biology and Technology 75: 24-27.

Sarwono, E., M. Nurdin, dan J. Prasetyo. 2013. Pengaruh kitosan dan Trichoderma sp. terhadap keparahan penyakit antraknosa (Colletotrichum capsici (Syd.) Butl. et Bisby) pada buah cabai (Capsicum annuum L.). Jurnal Agrotek Tropika 1(3): 336 – 340.

Soeka, Y.S., dan S. Sulistiani. 2012. Seleksi, karakterisasi, dan identifikasi bakteri penghasil kitinase yang diisolasi dari gunung Bromo Jawa Timur. Jurnal Natur Indonesia 13(2): 155-161.

Suptijah. P., A.M. Jacoeb, dan D. Rachmania. 2011. Karakteristik nano kitosan cangkang udang vannamei (Litopenaeus vannamei) dengan metode gelasi ionik. Jurnal Pengolahan Hasil Perikanan Indonesia 14(2):78-84.

Suryadi, Y., D.N. Susilowati, P. Lestari, T.P. Priyatno, I.M. Samudra, N. Hikmawati, and N.R. Mubarik. 2014. Characterization of bacterial isolates producing chitinase and glucanase for biocontrol of plant fungal pathogens. Journal of Agricultural Technology 4: 983-999.

Suryadi, Y., D.N. Susilowati, I.M. Samudra, and T.P. Priyatno. 2019. Pengaruh aplikasi kitosan antifungi untuk pengendalian penyakit antraknosa pada cabai. Jurnal Pertanian Tropik 13: 108–118.

Suryadi, Y., D.N. Susilowati, J. Kosasih, and S. Aminah. 2019. Characterization of chili philosphere and endophytics bacteria to control anthracnose disease. unpublished data.

Vishu Kumar, A.B., M.C. Varadaraj, L.R. Gowda, and R.N. Tharanathan. 2007. Low molecular weight chitosans-preparation with the aid of pronase, characterization and their bactericidal activity towards Bacillus cereus and Escherichia coli. Biochimica et Biophysica Acta 770 (4):495-505.