Pengaruh waktu ekstraksi menggunakan ultrasonic assisted extraction terhadap antosianin jantung pisang (Musa spp)

Dianasri Widyapuri; Ike Sitoresmi Mulyo Purbowati; Condro Wibowo

doi:10.21107/agrointek.v16i2.12559

Pengaruh waktu ekstraksi menggunakan ultrasonic assisted extraction terhadap antosianin jantung pisang (Musa spp)

Dianasri Widyapuri, Ike Sitoresmi Mulyo Purbowati, Condro Wibowo

Abstract

Banana blossom is a source of anthocyanin that is potentially utilized as natural dyes in the food industry. Anthocyanin from banana blossom can be extracted using Ultrasonic Assisted Extraction (UAE) and maceration method. Extraction using ultrasonic causes cavitation effect thereby provide an efficiency of extraction time if compared with maceration method. This study aimed to determine the effect of the duration of sonication treatment on total anthocyanin in banana blossom extract. Experimental design applied in this research is randomized block design with five treatments: maceration for 24 hours, sonication: 5 minutes, 10 minutes, 15 minutes and 20 minutes. Sonication was carried out at 40 kHz. Parameters determined were anthocyanin content, color (L*, a*, b*), morphological structure and total soluble solid. The results showed that sonication time did not significantly affect the total anthocyanin of banana blossom extract. Total anthocyanin of banana blossom extract with sonication time treatment ranged from 86.32 mg/L to 87.31 mg/L. The selected treatment for banana blossom extraction using UAE was 5 minutes sonication. This treatment resulted in a total anthocyanin concentration of 87.31 mg/L, which has comparable to the 24 hours maceration method. The color parameter (L*, a*, b*) and total soluble solid of the banana blossom extract with 5 minutes sonication was 28.67, 12.54, 17.92 and 8.32 %Brix, respectively. Meanwhile, the morphological structure showed formation of pores on the surface of banana blossom cells.

Keywords

banana blossom; anthocyanin; ultrasonic assisted extraction; sonication

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References

Abdulloh, M.A., Wardie, J., & Berkat, A.P. (2019). Analisis Usaha Tani Pisang Kepok di Desa Bangun Harja Kecamatan Seruan Hilir Timur. Socio Economics Agricultural, 14(2), 48-56.

Alvionita, J., Darwis, D., & Efdi, M. (2016). Ekstraksi dan Identifikasi Senyawa Antosianin dari Jantung Pisang Raja (Musa X paradisica L.) Serta Uji Aktivitas Antioksidannya. Jurnal Riset Kimia, 9(2), 21-28. https://doi.org/10.25077/jrk.v9i2.284

Amperawati, S., Hastuti, P., Pranoto, Y.,& Santoso, U. (2019). Efektifitas Frekuensi Ekstraksi Serta Pengaruh Suhu dan Cahaya Terhadap Antosianin dan Daya Antioksidan Ekstrak Kelopak Rosella (Hibiscus sabdariffa L.). Jurnal Aplikasi Teknologi Pangan, 8(1), 38–45. https://doi.org/10.17728/jatp.3527

Begum, Y. A., & Deka, S. C. (2017). Stability of spray-dried microencapsulated anthocyanins extracted from culinary banana bract. International Journal of Food Properties, 20(12), 3135–3148. https://doi.org/10.1080/10942912.2016.1277739

Di Khanh, N. (2015). Advances in the extraction of anthocyanin from vegetables. Journal of Food and Nutrition Sciences, 3(1), 126. https://doi.org/10.11648/j.jfns.s.2015030102.34

Djaeni, M., Ariani, N., Hidayat, R., & Utari, F. D. (2017). Ekstraksi Antosianin dari Kelopak Bunga Rosella (Hibiscus sabdariffa L.) Berbantu Ultrasonik: Tinjauan Aktivitas Antioksidan. Jurnal Aplikasi Teknologi Pangan, 6(3), 148-151.

https://doi.org/10.17728/jatp.236

Gajic, I. S., Savic, I., Boskov, I., Žerajić, S., Markovic, I., & Gajic, D. (2019). Optimization of ultrasound-assisted extraction of phenolic compounds from black locust (Robiniae pseudoacaciae) flowers and comparison with conventional methods. Antioxidants, 8(8), 1–14. https://doi.org/10.3390/antiox8080248

Giusti, M. M, & Wrolstad, R. E. (2001). Characterization and measurement of anthocyanins by uv-visible spectroscopy. Current Protocol in Food Analitical Chemistry, F1.2.1-F1.2.13. https://doi.org/10.1002/0471142913.faf0102s00

Ince, A. E., Sahin, S., & Sumnu, G. (2012). Comparison of microwave and ultrasound-assisted extraction techniques for leaching of phenolic compounds from nettle. Journal of Food Science and Technology, 51(10), 2776–2782. https://doi.org/10.1007/s13197-012-0828-3

Ismawati, N., Nurwantoro & Pramono, Y.B. (2016). Nilai pH dan Total Padatan Terlarut dengan Penambahan Ekstrak Bit (Beta Vulgaris L.). Jurnal Aplikasi Teknologi Pangan, 5(3), 89–93. https://doi.org/10.17728/jatp.181

Ismed, S., Kesuma, & Andini, F. (2017). Pengaruh Suhu dan Lama Penyimpanan terhadap Indikator Film dari Ekstrak Kelopak Bunga Rosella (Hibiscus sabdariffa L.) sebagai Smart Packaging untuk Mendeteksi Kerusakan Nugget Ayam. Jurnal Aplikasi Teknologi Pangan, 6(4), 167-172. https://doi.org/10.17728/jatp.267

Kumar, K., Srivastav, S., & Sharanagat, V. S. (2021). Ultrasound assisted extraction (UAE) of bioactive compounds from fruit and vegetable processing by-products: A review. Ultrasonics Sonochemistry, 70, 105325. https://doi.org/10.1016/j.ultsonch.2020.105325

Lau, B. F., Kong, K. W., Leong, K. H., Sun, J., Wang, Z., Mustafa, M. R., Ling, T. C., Ismail, A., & He, X. (2020). Banana inflorescence: Its bio-prospects as an ingredient for functional foods. Trends in Food Science & Technology, 97, 14-28. https://doi.org/10.1016/j.tifs.2019.12.023

Lestario, L. N., Lukito, D., & Timotius, K. H. (2009). Kandungan Antosianin dan Antosianidin Dari Jantung Pisang Klutuk (Musa brachycarpa Back) dan Pisang Ambon (Musa acuminate Colla). Jurnal Teknologi dan Industri Pangan, 20(2): 143-148.

Lestario, L. N., Yoga, M. K. W. C., & Kristijanto, A. I. (2014). Stabilitas Antosianin Jantung Pisang Kepok (Musa paradisiaca L) Terhadap Cahaya Sebagai Pewarna Agar-Agar. Agritech, 34(4): 374-381. https://doi.org/10.22146/agritech.943

Ove, T. A., Kamal, M. M., Nasim, S. M. N. I., Momin, M. M. I., & Mondal, S. C. (2019). Extraction and quantification of anthocyanin from banana bracts using different pH and solvent concentration. International Journal, 4(2), 60–64.

Panda, D., & Manickam, S. (2019). Cavitation technology-the future of greener extraction method: A review on the extraction of natural products and process intensification mechanism and perspectives. Applied Sciences (Switzerland), 9(4). 766. https://doi.org/10.3390/app9040766

Pourcel, L., Irani, N. G., Lu, Y., Riedl, K., Schwartz, S., & Grotewold, E. (2010). The formation of anthocyanic vacuolar inclusions in arabidopsis thaliana and implications for the sequestration of anthocyanin pigments. Molecular Plant, 3(1), 78–90. https://doi.org/10.1093/mp/ssp071

Preethi, P., & Balakrishnamurthy, G. (2011). Assessment of banana cultivars for pigment extraction from bracts, its suitability and stability as food colourant. International Journal of Processing and Post Harvest Technology, 2(2), 98–101.

Puspawati, G. A. K. D., Marsono, Y., Supriyadi, & Armunanto, R. (2018). Comparison of sonication with maceration on antioxidant potency of anthocyanin and karotenoid of tamarillo (Solanum betaceaum Cav.). Agritech, 38(3): 304-312. https://doi.org/10.22146/agritech.28959

Rodriguez-Amaya, D. B. (2019). Update on natural food pigments - A mini-review on carotenoids, anthocyanins, and betalains. Food Research International, 124(2017), 200–205. https://doi.org/10.1016/j.foodres.2018.05.028

Sartika, W., Rahmi, E., Indrayani, I., Wati, R., & Suresti, A. (2018). Perbaikan Kemasan pada Dendeng Jantung Pisang pada Asosiasi Wanita Tani (ASWATA) di Kabupaten Padang Pariaman. Conference on Innovation and Application of Science and Technology (CIASTECH 2018), 223-227.

Sholihah, M. Ahmad, U., & Budiastra, I. W. (2017). Aplikasi Gelombang Ultrasonik untuk Meningkatkan Rendemen Ekstraksi dan Efektivitas Antioksidan Kulit Manggis. Jurnal Keteknikan Pertanian, 5(2), 161-168. https://doi.org/10.19028/jtep.05.2.161-168

Singh, S., Gaikwad, K. K., & Lee, Y. S. (2018). Anthocyanin–A natural dye for smart food packaging systems. Korean Journal of Packaging Science & Technology, 24(3), 167-180. https://doi.org/10.20909/kopast.2018.24.3.167

Widyasanti, A., Nurlaily, N., & Wulandari, E. (2018). Karakteristik Fisikokimia Antosianin Ekstrak Kulit Buah Naga Merah Menggunakan Metode UAE. Jurnal Ilmiah Rekayasa Pertanian dan Biosistem, 6(1), 27-38. https://doi.org/10.29303/jrpb.v6i1.63

Winata, E.W., & Yunianta. (2015). Ekstraksi Antosianin Buah Murbei ( Morus alba L .) Metode Ultrasonic Bath (Kajian Waktu dan Rasio Bahan : Pelarut). Jurnal Pangan Dan Agroindustri, 3(2), 773–783.

Yuniati, Y., Elim, P. E., Alfanaar, R., Kusuma, H. S., & Mahfud. (2021). Extraction of anthocyanin pigment from Hibiscus sabdariffa I. by ultrasonic-assisted extraction. IOP Conference Series: Materials Science and Engineering, 1010(1). https://doi.org/10.1088/1757-899X/1010/1/012032

Zhu, Z., Guan, Q., Guo, Y., He, J., Liu, G., Li, S., Barba, F. J., & Jaffrin, M. Y. (2016). Green ultrasound-assisted extraction of anthocyanin and phenolic compounds from purple sweet potato using response surface methodology. Int. Agrophys., 30, 113-122. https://doi.org/ 10.1515/intag-2015-0066.