KAJIAN PENGGUNAAN KULIT BUAH MELINJO (Gnetum gnemon) SEBAGAI SUMBER ENZIM PROTEASE

R. A. Sidqi Zaed Z.M.

Abstract


The research consist of six parts.  These studies aim at : (1) seeking an accurate of extraction–isolation methode in peel of Gnetum gnemon, (2) determining pH optimum enzyme protease in peel of Gnetum gnemon, (3) determining the optimum temperature of enzyme protease in peel of Gnetum gnemon, (4) knowing the activity of enzyme in several concentrations, (5) knowing kind of enzyme based on its active, and (6) knowing the stability of enzyme to high temperature in peel of Gnetum gnemon.

The research was done on February to September 2008  at Base Laboratory  of Trunojoyo University and Biochemestry Laboratory of Brawijaya University. 

The first research uses saturate sulate ammonium 50 % and 60 %, acetone 1:1 and ethanol 1:1.  Observation was done on enzyme activities resulted (µmol tir.ml-1.min-1) and its rendement (%).  Extraction methode by ammonium sulfate 60 % µmol tir.ml-1.min-1 is the best methode with activity 62.15 x 10- 2   µmol tir.ml-1.min-1 and yields 1.52 %.

The second research was examined in pH 4.0, 4.5, 5.0, 6.0, 7.0, 7.5, 8.0 and 8.5.  The research result shows that the optimum activity of enzyme protease in peel of Gnetum gnemon around pH neutral is 6.5 (60.33 x10- 2   µmol tir.ml-1.min-1).

The third research was experimented on temperature 30, 35, 40, 45, 50, 60, 65 and 70 degrees Celsius.  The research result shows that enzyme protease which extracted in peel of Gnetum gnemon  indicates the highiet activity 40 degrees Celcius with activity 63.94 x 10- 2   µmol tir.ml-1.min-1. The fourth research uses substrate casein 0.2, 0.4, 0.6, 0.8, 1.0, 1.2, 1.4 and 1.6 % (b/v).  The research concludes that concentration of substrate casein 0.8 % shows a saturate.  The highest activity value is 63.49 x 10- 2   µmol tir.ml-1.min-1.

The fifth research shows that the highest enzyme activity becaused of the influences HCN (64.26 x    10- 2   µmol tir.ml-1.min-1.  Therefore, it can be concluded that enzyme protease in peel of Gnetum gnemon is sulfidril (sulfide).

The sixth research shows that the stability endures until 50 degrees Celcius (59.84 x 10- 2   µmol tir.ml-1.min-1.  It means that enzyme protease in peel of Gnetum gnemon is kind of enzyme protease with high temperature stability.

References


Abe, H., Asakura, T., Watanabe, H, and Arai. 1997. Oryzasin as an Aspartic Proteanase Occuring in Rice Seeds : Purification, Characterisation an Application to Milk Clitting. J. Agric. Food Chem., 45 (4), 1070 – 1075.

Chairunnisa, H. 1988. Isolasi Enzim Bromelin Kasar dari Bonggol Nenas. Simposium Bioproses dalam Industri Pangan. Pusat Antar Universitas Bioteknologi. IPB. Bogor.

Chinas, F.A.I and Canales, A.L.M. 1986. Proteolytic Enzym from Cnidosculus chayamansa “chaya”. J. Food Sci., 51 (1), 243 – 244.

Diaz, C.P. 2007. Gnetum gnemon and Its Prospect in Agroforestry. Google. 25 Pebruari 2007.

Indarto, C. 2006. Aktivitas Protease Kulit Buah Belinjo (Gnetum gnemon). Agrointek. 1 (I), 34 – 39.

Noda, K,., Koyanagi, M and Kayami, C. 1994. Purification and Characterisation of an Endoprotease from Melon Fruit. J. Food Sci., 59 (3), 585 – 587.

Sanogo, T. Paquet and Linden. 1990. Proteolysis Casein by Papain in a Complex Environment Influece of Ionic Strenth on The Reaction Product. J. Food Sci., 55 (3), 796 – 800.

Schwimmer, S. 1981. Source Book of Food Enzymology. The Avi Publishing Company, Inc. Westport, Conecticut, USA.

Suhartono, M.T. 1992. Protease. Pusat Antar Universitas IPB. Bogor.

Whitaker, J.R. 1994. Principle of Enzymology for The Food Science. Second Edition. Marcel Decker. New York.




DOI: https://doi.org/10.21107/agrovigor.v3i1.256

Refbacks

  • There are currently no refbacks.


Copyright (c) 2015 R. A. Sidqi Zaed Z.M.

Creative Commons License
This work is licensed under a  Creative Commons Attribution-ShareAlike 4.0 International License
Admin Jefri Bocorkan Momen Petir Zeus Muncul di Gates of Olympus KASTATOTO Gates of Olympus Kembali Dibicarakan, Jefri Sebut Banyak Pemain Menang di KASTATOTO Pemain Surabaya Ikuti Saran Jefri Coba Gates of Olympus di KASTATOTO, Ini Hasilnya Keseruan Gates of Olympus Bikin Jefri Rekomendasikan Game Ini di KASTATOTO Jefri Sebut Pemain Makassar Paling Aktif Main Gates of Olympus di KASTATOTO Gates of Olympus Bikin Heboh Lagi, Jefri Ulas Hadiah Besar di KASTATOTO Admin Jefri Tunjukkan Fitur Menarik dalam Gates of Olympus, Ini Versi KASTATOTO Kenapa Jefri Lebih Pilih Gates of Olympus? Ini Pengalamannya di KASTATOTO Petir Zeus Berkali-kali Muncul, Jefri Bagikan Tangkapan Layar di KASTATOTO Jefri Ajak Pemain Yogyakarta Ramaikan Gates of Olympus Lewat KASTATOTO Admin Jefri Ungkap Game PG Soft Paling Seru yang Bisa Dicoba di BUKTI4D Pengalaman Admin Jefri Mainkan PG Soft di BUKTI4D, Ternyata Begini Rasanya Jefri Sarankan Game PG Soft Ini ke Pemain Jakarta, Cek Sendiri di BUKTI4D PG Soft Punya Fitur Baru, Begini Kata Admin Jefri Soal Perubahannya di BUKTI4D Pemain Bandung Ikuti Rekomendasi Jefri dan Mainkan Game PG Soft di BUKTI4D Apa Kata Admin Jefri Soal Tren Game PG Soft di BUKTI4D? Banyak Pemain Kaget! Fitur Tersembunyi dalam PG Soft Akhirnya Diulas Admin Jefri dari BUKTI4D Game PG Soft Ini Bikin Admin Jefri Betah Main di BUKTI4D, Apa Istimewanya? Jefri Beberkan Alasan Kenapa Game PG Soft Lebih Diminati di BUKTI4D BUKTI4D Umumkan Event Game PG Soft, Admin Jefri Ajak Pemain Ikut Meriahkan

slot gacor

Nonton Indonesia vs Australia Sambil Spin Mahjong Ways 2 Auto Cuan Habis Sorak Gol Indonesia Langsung JP di Gates of Olympus Serunya Indonesia vs Australia dan Jackpot Mahjong Wins 3 di Waktu yang Sama Dukung Indonesia Lawan Australia Sambil Cuan dari Mahjong Ways 2 Gates of Olympus Gacor Saat Indonesia Cetak Gol ke Gawang Australia Timnas Indonesia Berjuang, Aku Juga Spin Mahjong Wins 3 Demi JP Gol Indonesia di Menit Akhir dan JP Mahjong Ways 2 Datang Bersamaan Sambil Nunggu Kick-off Indonesia, Spin Gates of Olympus Dulu Pertandingan Indonesia Bikin Tegang, Mahjong Ways 2 Bikin Saldo Tenang Nonton Indonesia Bersama Teman, Spin Mahjong Wins 3 Bersama Cuan Bocoran Pola Terbaru Jackpot Terbesar Mahjong Ways di Jewel4D, Trik Ampuh Auto Maxwin Cara Gampang Jackpot Slot Mahjong Ways di Jewel4D dengan Pola Terbaru Trik Bermain Slot Mahjong Ways Jewel4D dengan Pola Maxwin Terupdate Pola Bermain Slot Mahjong Ways di Jewel4D agar Jackpot Terbesar Lebih Gampang Bocoran Jitu Pola Maxwin Slot Mahjong Ways Terbaru di Jewel4D Hari Ini Cara Mendapatkan Jackpot Slot Mahjong Ways di Jewel4D dengan Trik Bermain Terbaik Strategi Bermain Slot Mahjong Ways di Jewel4D Biar Lebih Gampang Jackpot Trik dan Tips Slot Mahjong Ways Maxwin dengan Pola Gampang Menang di Jewel4D Tips dan Trik Slot Mahjong Ways Jewel4D Terbaru untuk Jackpot Maxwin Bocoran Gacor Slot Mahjong Ways Jewel4D dengan Trik dan Pola Jackpot