VALIDASI BAKTERI PELARUT FOSFAT INDIGENUS MADURA PADA RHIZOSFER
Abstract
ABSTRACT
Often generalized that the determination effectiveness of biological agents depends on environment. The purpose of this study is to obtain an explanation of the role of growth limiting factors, chemical or physical on the effectiveness Madura indigenous Pseudomonas aeruginosa as a phosphate solubilizing. The method used in achieving the goal of research is, perform in vitro studies on the type of carbon source (sugar) and the level of salinity as the growth limiting factors of bacteria on the effectiveness of phosphate dissolution and study the influence of the the rice rhizosphere type of phosphate available. Found that in vitro, the type of carbon source and salinity levels affect phosphate concentrations and abundance of bacterial cells. Increased levels of salinity decreases the abundance of bacterial cells. Average cell population fluorescent pseudomonads in fields rice rhizosphere is higher than the lowland rice rhizosphere, the cell population fluorescent pseudomonads higher level provide higher available phosphate and vegetative growth of rice better.
Key words: Phosphate solubilizing, limiting growth, carbon source, salinity, the rhizosphere Madura indigenous.Full Text:
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Atlas, R. M. 2004. Handbook of Microbiological Media Third Edition. Ann Arbor. MI: CRC Press.
Bagyaraj. D. J, P. U. Krishnaraj, S. P. S. Khanuja. 2000. Mineral phosphate solubilization: Agronomic implications, mechanism and molekular genetics. Proc. Ind. Nat. Sci. Acad. (PINSA) B66 Nos 2 & 3. 69-82
Benson, H. J. 1990. Microbiological Applications A Laboratory Manual in General Microbiology Fifth Edition. Dubuque. Wm.C.Brown Publishers, hlm. 52-54.
Brimecombe, M. J. , F. A. De Leij, J. M. Lynch. 2001. The effect of root exudates on rhizosphere microbial population di dalam The Rhizosphere Biochemistry and Organik substances at the Soil-Plant Interface. (ed. Pinton, R., Z. Varanini, P. Nannipieri). New York. Marcel Dekker. 95-140.
Fankem, H., L. Ngo Nkot, A. Deubel, J. Quinn, W. Merbach, F. X. Etoa, D. Nwaga. 2008. Solubilization of inorganic phosphates and plant growth promotion by strains of Pseudomonas fluorescens isolated from acidic soil of Cameroon. Afric. J. Microbiol. Research 2: 171-178.
Illmer. P., F. Schinner. 1995. Solubilization of inorganic calcium phosphate- Solubilization mechanisms. Soil Biol. Biochem. 7: 257-263.
Khan, A. A., G. Jilani, M. S. Akhtar, S. M. S. Naqvi, M. Rasheed. 2009. Phosphorus solubilizing bacteria: Occurrance, mechanisms and their role in crop production. J. Agric. Biol. Sci. 1: 48-58.
Khan, J. A. , R. M. Bhatnagar. 1997. Studies on solubilization of insoluble phosphate rocks by Aspergillus niger and and Penicillium sp. Fertil Technol. 14:329-333.
Pawana, G., M. Tripatmasari, A. Supriyanto. 2010. Pengembangan Teknologi Sarana Produksi Pengganti Untuk Budidaya Tembakau Madura. Riset Unggulan 2009 Universitas Trunojoyo. Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Trunojoyo, hlm. 19-31.
Pawana, G., Syekhfani, T. Surtiningsih,W. S. Wahyuni. 2013. Fluorescent pseudomonad Madura indigenous phosphate solubilizing and their behavior of phosphate solubilization. Asian J. Exp. Biol. Sci. 4: 524-531.
Prasetyo, B. H., D. Santoso, L. R. Widowati. 2005. Petunjuk Teknis Analisa Kimia Tanah, Tanaman, Air dan Pupuk. Balai Penelitian Tanah. Badan Penelitian dan Pengembangan Pertanian. Jakarta. Departemen Pertanian.
Reyes, I, A. Valery, Z. Valduz. 2007. Phosphate solubilizing microorganism isolate from rhizospheric and bulk soil of colonizer plants at an abandoned rock phosphate mine. Di dalam E. Velazquez dan C. Rodriguez-Barrueco (edt). First International Meeting on Microbial Phosphate Solubilization. 69-75.
Rodriguez, H., R. Fraga. 1999. Phosphate Solubilizing bacteria and their role in plant growth promotion. Biotechnology Advances 17 (1999): 319-339.
Vega, N. W. O. 2007. A review on beneficial effects of rhizosphere bacteria on soil nutrient availability and plant nutrien uptake. Rev. Fac. Nal. Agr. Medellin 60: 3621-3643.
Vyas, P., A. Gulati. 2009. Organic acid production in vitro and plant growth promoting in maize under controlled environment by phosphate-solubilizing fluorescent Pseudomonad. BCM Microbiology 9: 174.
DOI: https://doi.org/10.21107/agrovigor.v7i2.1444
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