PENENTUAN KLORAMFENIKOL DALAM DAGING AYAM BROILER DENGAN METODE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC)

Aman Sentosa Panggabean

doi:10.21107/rekayasa.v5i1.2105

PENENTUAN KLORAMFENIKOL DALAM DAGING AYAM BROILER DENGAN METODE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC)

Aman Sentosa Panggabean

Abstract

Determination of chloramphenicol on broiler meat in the traditional market by using High-Performance Liquid Chromatography (HPLC) method has been done. The optimal of result determination to obtain, the HPLC system has been optimized for the important parameters are a composition of the mobile phase, injection sample volume, flow rate, and even pH. The result of research shown that the optimum conditions to obtain from the research are a composition of mobile phase methanol: water (80: 20), injection sample volume 5 μL, flow rate 0.5 mL/minute and eluent pH are 6 has been achieved. The analytical performance of the method has been optimized and gives a good performance which shown with reproducibility of this method as the coefficient of variation percentage (% CV) was 0.2198 %, limit of detection was 0.1051 mg/L, and % recovery for the chicken meat sample with 3 different samples more than 95 %. This result showed this method capable of an application for the routine analysis of the determination chloramphenicol content in samples

Keywords

Chloramphenicol; HPLC; Analytical performance; Broiler Meat;

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