Pengaruh Manipulasi Ploidi Terhadap Efektivitas Pertumbuhan dan Hasil Tanaman Labu Kuning (Cucurbita moschene Dutchene)

Reza Prakoso Dwi Julianto, I Made Indra Agastya, Wahyu Fikrinda

Abstract

Pumpkin (Cucurbita moschene Duthene) is plant sources of food that has high nutrition content. Increasing the productivity of pumpkin can be done through programs, which is the ploidy manipulation technique, the technique using a mutagen, namely colchicine. Application of colchicine in the wrong dose and soaking time can be toxic to plants. The research aim to gain recommendations for the right concentration of colchicine immersion to increase the growth and yield of pumpkin plants. Research was done by using a split plot design with subplots with colchicine concentration and the main plots of local Malang pumpkin varieties. The research results that application of colchicine immersion in several local pumpkin varieties showed a significant relationship at fruit quantity parameter. Application of colchicine with 3 dose ppm on local pumpkin variety Malang 1 (P3V1) showed the highest average fruit quantity compared to other treatments, which was 4 pieces. Optimal concentration of colchicine to increase the yield of local pumpkin plants is with 3 dose ppm can be seen from the highest average value on several parameters among others fruit number, fruit weight, and fruit diameter, but not significantly different from the colchicine concentration of 2 ppm and 4 ppm. The colchicine concentration of 2 ppm showed the highest average value of the sex ratio parameter was 25.51%.

Keywords

colchicine, ploidi manipulation, pumpkin, sex ratio

References

Abdoli, M., Moieni, A., & Naghdi Badi, H. (2013). Morphological, physiological, cytological and phytochemical studies in diploid and colchicine-induced tetraploid plants of Echinacea purpurea (L.). Acta Physiologiae Plantarum, 35(7), 2075–2083.

Amiri, S., Kazemitabaar, S. K., Ranjbar, G., & Azadbakht, M. (2010). the Effect of Trifluralin and Colchicine Treatments on Morphological Characteristics of Jimsonweed (Datura Stramonium L.). Trakia Journal of Sciences, 8(4), 47–61.

Deninta, N., Onggo, T. M., & Kusumiyati, K. (2017). Pengaruh Berbagai Konsentrasi dan Metode Aplikasi Hormon GA3 terhadap Pertumbuhan dan Hasil Tanaman Brokoli Kultivar Lucky di Lembang. Indonesian Journal of Applied Sciences, 7(2), 9–14.

Gantait, S., Mandal, N., Bhattacharyya, S., & Das, P. (2011). Induction and identification of tetraploids using in vitro colchicine treatment of Gerbera jamesonii Bolus cv. Sciella. Plant Cell Tissue and Organ Culture, 106, 485–493.

Haryanti, S., Hastuti, R. B., Setiari, N., & Banowo, A. (2009). Pengaruh Kolkisin Terhadap Pertumbuhan, Ukuran Sel Metafase dan Kandungan Protein Tanaman Kacang Hijau (vigna radiata (L) wilczek). Jurnal Penelitian Sains & Teknologi, 10(2), 112–120.

Human, S., Loekito, S., Trilaksono, M., & Syaifudin, A. (2016). Pemuliaan Mutasi Tanaman Nanas ( Ananas comosus ( L .) Merr .) Menggunakan Iradiasi Gamma untuk Perbaikan Varietas Nanas Smooth Cayenne. Jurnal Ilmiah Aplikasi Isotop dan Radiasi, 12(1), 13–22.

Julianto, R. P. D., & Sumiati, A. (2017). Keragaman Fenotipe dan Produktivitas Labu Kuning Nusantara (Cucurbita moschene Dutchene) dalam Rangka Pengembangan Varietas Unggul. Buana Sains, 17(2), 137–142.

Kazi, N. (2015). Polyploidy in Solanaceous Crops. Asian journal of multidisciplinary studies, 3.

Napitupulu, D., & Winarto, L. (2010). Pengaruh Pemberian Pupuk N Dan K Terhadap Pertumbuhan Dan Produksi Bawang Merah. Jurnal Hortikultura, 20(1), 27–35.

Rahayu, E. M. Della, Sukma, D., Syukur, M., & Aziz, S. A. (2015). Induksi Poliploidi Menggunakan Kolkhisin Secara In Vivo Pada Bibit Anggrek Bulan ( Phalaenopsis amabilis ( L .) BLUME ). Buletin Kebun Raya, 18(1), 41–48.

Rayburn, A. L., Kushad, M. M., & Wannarat, W. (2008). Intraspecific genome size variation in pumpkin (Cucurbita pepo subsp. pepo). HortScience, 43(3), 949–951.

Santoso, E. B., Basito, & Rahadian, D. (2013). Pengaruh Penambahan Berbagai Jenis Susu Terhadap Sifat Sensoris dan Fisikokimia Puree Labu Kuning (Cucurbita moschata ). Teknosains Pangan, 2(3), 51.

Sartika, T. V., & Basuki, N. (2017). Pengaruh Konsentrasi Kolkisin Terhadap Perakitan Putative Mutan Semangka ( Citrullus lanatus ). Jurnal Produksi Tanaman, 5(10), 1669–1677.

Sirojuddin, Rahayu, T., & Laili, S. (2017). Pengaruh Pemberian Berbagai Konsentrasi Kolkisin dan Lama Perendaman terhadap Respon Fenotipik Zaitun (Olea europaea). Biosaintropis, 2(2), 36–41.

Sitompul, S., & Guritno, B. (1995). Analisa Pertumbuhan Tanaman. Gadjah Mada University Press.

Sobir, & Siregar, D. (2014). Budidaya Melon Unggul. Penebar Swadaya.

Soetopo, L., & Hosnia, D. (2018). In vivo polyploid-induction by colchicine on orchids phalaenopsis pulcherrima (Lindl.) J.J Smith. Bioscience Research, 15, 941–949.

Suharni, S. (2004). Evaluasi Morologi, Anatomi, Fisiologi Dan Sitologi Tanaman Rumput Pakan Yang Medapat Perlakuan Kolkhisin. Tesis.

Sumarni, N., Rosliani, R., Basuki, R. S., & Hilman, Y. (2012). Respons Tanaman Bawang Merah terhadap Pemupukan Fosfat pada Beberapa Tingkat Kesuburan Lahan (Status P-Tanah). Jurnal Hortikultura, 22(2), 130.

Sumiati, E., & Gunawan, O. (2007). Aplikasi Pupuk Hayati Mikoriza Untuk Meningkatkan Efisiensi Serapan Unsur Hara NPK Serta Pengaruhnya Terhadap Hasil Dan Kualitas Umbi Bawang Merah. Jurnal Hortikultura, 17(1), 34–42.

Suminah, Sutarno, & Setyawan, A. D. (2002). Polyploid induction of Allium ascalonicum L. by colchicine. Biodiversitas Journal of Biological Diversity, 3(1), 174–180.

Suranto. (2001). Studies on Ranunculus Population: Isozymic Pattern. Biodiversitas Journal of Biological Diversity, 2(1), 85–91.

Suryo. (2007). Sitogenetika (Cetakan ke). Gadjah Mada University Press.

Tedianto. (2012). Karakterisasi Labu Kuning (Cucurbita moschata) Berdasarkan Penandan Morfologi dan Kandungan Protein, Karbohidrat, Lemak pada Bebagai Ketinggian Tempat. Tesis.

Trojak-Goluch, A., & Skomra, U. (2013). Artificially induced polyploidization in Humulus lupulus L. and its effect on morphological and chemical traits. Breeding Science, 63(4), 393–399.

Widiyanti, Suranto, & Sugiyanto. (2008). Studi Variasi Morfologi Biji, Serbuk Saridan Pola Pita Isozim Padi (Oryza sativa) Varietas Rojolele. Bioteknologi, 5(1), 18–25.

Yulianti, F., Purwito, A., Husni, A., & Dinarti, D. (2015). Induksi Tetraploid Tunas Pucuk Jeruk Siam Simadu (Citrus nobilis Lour) Menggunakan Kolkisin secara In Vitro. Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy), 43(1), 66.

Zulfahmi, Suranto, & Mahajoeno, E. (2015). Karakteristik tanaman labu kuning (Cucurbita moschata) berdasarkan penanda morfologi dan pola pita isozim peroksidase. Prosiding Seminar Nasional Biotik 2015, 266–273.

DOI

https://doi.org/10.21107/rekayasa.v16i1.11895

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