Pengaruh Ekstraksi Hijau Menggunakan NADES Terhadap Karakteristik Ekstrak Daun Tua Mangrove Avicennia marina

Desy Arisandi, Hartati Kartikaningsih, Heder Djamaludin, Febrian Salim Prasetyo, Mirza Gulam Ahmad, Jeny Ernawati Tambunan, Gilang Rusrita Aida, Kristina Marsela

Abstract


Ekstraksi hijau menggunakan Natural Deep Eutectic Solvent (NADES) berpotensi meningkatkan efisiensi perolehan senyawa bioaktif dari bahan mangrove. Penelitian ini bertujuan menganalisis pengaruh variasi rasio mol NADES asam sitrat:glukosa terhadap karakteristik ekstrak daun tua mangrove Avicennia marina, meliputi kadar air simplisia, rendemen, pH, dan profil fitokimia kualitatif. Penelitian menggunakan Rancangan Acak Lengkap nonfaktorial dengan empat perlakuan rasio mol (1:1, 2:1, 3:1, 4:1) dan empat ulangan. Simplisia dibuat dari daun tua, kemudian diekstraksi menggunakan NADES, diikuti pengukuran rendemen dan pH serta skrining fitokimia (alkaloid, flavonoid, saponin, tanin, steroid, terpenoid). Data rendemen dan pH dianalisis menggunakan ANOVA dan uji Tukey (α=5%). Kadar air simplisia sebesar 8,40±0,53%. Rendemen meningkat seiring kenaikan rasio asam sitrat dengan nilai tertinggi pada 4:1 (76,90±0,47%) dan terendah pada 1:1 (74,60±1,10%). pH ekstrak menurun dari 1,88±0,02 (1:1) menjadi 1,51±0,02 (4:1). Skrining fitokimia menunjukkan flavonoid, tanin, dan alkaloid terdeteksi pada semua perlakuan. Rasio 4:1 direkomendasikan sebagai kondisi awal untuk standardisasi ekstrak dan penelitian lanjutan berbasis kuantifikasi dan bioaktivitas.

References


Aktaş, H., & Kurek, M. A. (2024). Deep eutectic solvents for the extraction of polyphenols from food plants. Food Chemistry, 444, 138629. https://doi.org/10.1016/j.foodchem.2024.138629

Chevé-Kools, E., Choi, Y. H., Roullier, C., Ruprich-Robert, G., Grougnet, R., Chapeland-Leclerc, F., & Hollmann, F. (2025). Natural deep eutectic solvents (NaDES): Green solvents for pharmaceutical applications and beyond. Green Chemistry, 27(28), 8360–8385. https://doi.org/10.1039/D4GC06386D

ElDohaji, L. M., Hamoda, A. M., Hamdy, R., & Soliman, S. S. (2020). Avicennia marina a natural reservoir of phytopharmaceuticals: Curative power and platform of medicines. Journal of Ethnopharmacology, 263, 113179. https://doi.org/10.1016/j.jep.2020.113179

Godlewska, K., Pacyga, P., Piel, W., & Michalak, I. (2022). Methods for rapid screening of biologically active compounds present in plant-based extracts. Molecules, 27(20), 7094. https://doi.org/10.3390/molecules27207094

Hayat, J., Akodad, M., Moumen, A., Baghour, M., Skalli, A., Ezrari, S., & Belmalha, S. (2020). Phytochemical screening, polyphenols, flavonoids and tannin content, antioxidant activities and FTIR characterization of Marrubium vulgare L. from 2 different localities of Northeast of Morocco. Heliyon, 6(11), e05609. https://doi.org/10.1016/j.heliyon.2020.e05609

Herrero, M. (2024). Towards green extraction of bioactive natural compounds. Analytical and Bioanalytical Chemistry, 416, 2039–2047. https://doi.org/10.1007/s00216-023-04969-0

Hikmawanti, N. P. E., Ramadon, D., Jantan, I., & Mun’im, A. (2021). Natural deep eutectic solvents (NADES): Phytochemical extraction performance enhancer for pharmaceutical and nutraceutical product development. Plants, 10(10), 2091. https://doi.org/10.3390/plants10102091

Ibrahim, H. A. H., Abdel-Latif, H. H., & Zaghloul, E. H. (2022). Phytochemical composition of Avicennia marina leaf extract, its antioxidant, antimicrobial potentials and inhibitory properties on Pseudomonas fluorescens biofilm. Egyptian Journal of Aquatic Research, 48(1), 53–60. https://doi.org/10.1016/j.ejar.2021.10.007

Kartikaningsih, H., Djamaludin, H., Fauziyah, J. N., Audina, N., Noviyanti, L., & Saputra, D. (2024). Green extraction of Avicennia marina leaves by natural deep eutectic solvents: Phytochemical profile, antioxidant activity, molecular docking and ADMET analysis. Rasayan Journal of Chemistry, 17, 1123–1133. https://doi.org/10.31788/RJC.2024.1738923

Liu, Y., Friesen, J. B., McAlpine, J. B., Lankin, D. C., & Pauli, G. F. (2018). Natural deep eutectic solvents: Properties, applications, and perspectives. Journal of Natural Products, 81(3), 679–690. https://doi.org/10.1021/acs.jnatprod.7b00945

Mišan, A., Nađpal, J., Stupar, A., Pojić, M., Mandić, A., Verpoorte, R., & Choi, Y. H. (2020). The perspectives of natural deep eutectic solvents in agri-food sector. Critical Reviews in Food Science and Nutrition, 60(15), 2564–2592. https://doi.org/10.1080/10408398.2019.1650717

Mitra, S., Naskar, N., Lahiri, S., & Chaudhuri, P. (2023). A study on phytochemical profiling of Avicennia marina mangrove leaves collected from Indian Sundarbans. Sustainable Chemistry for the Environment, 4, 100041. https://doi.org/10.1016/j.scenv.2023.100041

Rozirwan, Nugroho, R. Y., Hendri, M., Fauziyah, Putri, W. A. E., & Agussalim, A. (2022). Phytochemical profile and toxicity of extracts from the leaf of Avicennia marina (Forssk.) Vierh. collected in mangrove areas affected by port activities. South African Journal of Botany, 150, 903–919. https://doi.org/10.1016/j.sajb.2022.08.037

Socas-Rodríguez, B., Torres-Cornejo, M. V., Álvarez-Rivera, G., & Mendiola, J. A. (2021). Deep eutectic solvents for the extraction of bioactive compounds from natural sources and agricultural by-products. Applied Sciences, 11(11), 4897. https://doi.org/10.3390/app11114897

Vo, T. P., Ho, T. A. T., Truong, K. V., Ha, N. M. H., & Nguyen, D. Q. (2024). Combining novel extraction techniques with natural deep eutectic solvent to acquire phenolic and terpenoid compounds from Paris polyphylla roots. Journal of Agriculture and Food Research, 18, 101544. https://doi.org/10.1016/j.jafr.2024.101544

Zhou, P., Hu, H., Wu, X., Feng, Z., Li, X., Tavakoli, S., Wu, K., Deng, L., & Luo, H. (2025). Botany, traditional uses, phytochemistry, pharmacological activities, and toxicity of the mangrove plant Avicennia marina: A comprehensive review. Phytochemistry Reviews, 1–36. https://doi.org/10.1007/s11101-025-10080-2




DOI: https://doi.org/10.21107/juvenil.v7i2.33201

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Juvenil byJurusan Kelautan dan Perikananis licensed under a Creative Commons Attribution 4.0 International License.

Published by: Department of Marine and Fisheries, Trunojoyo University of Madura