Identifikasi Senyawa Metabolit Sekunder Flavonoid Pada Daging Ikan Buntal (Arothron manilensis)

Tresia Sanda Layuk, Ahmad Shofy Mubarak, Ratna Diyah Palupi, Rahmadani Rahmadani

Abstract


ABSTRAK

Senyawa metabolit sekunder merupakan senyawa yang diproduksi suatu makhluk hidup untuk mempertahankan kelangsungan hidup, sebagai bentuk upaya mempertahankan diri dari predator alaminya. Ikan buntal adalah satu organisme laut yang memiliki potensi dalam menghasilkan metabolit sekunder. Tujuan dari penelitian ini untuk menganalisis perbandingan larutan eluen dan fraksi yang optimal untuk menentukan senyawa metabolit sekunder flavonoid pada daging ikan buntal Arothron manilensis. Metode penelitian dilakukan menggunakan metode Skrining Fitokimia dan Kromatografi Lapis Tipis (KLT) untuk mendeteksi senyawa metabolit sekunder. Hasil penelitian menunjukkan bahwa penentuan eluen, kepolaran eluen maupun pereaksi yang digunakan sangat memengaruhi nampaknya profil senyawa metabolit sekunder. Pada fraksi etil asetat senyawa metabolit sekunder flavonoid terlihat paling kuat sedangkan pada fraksi n-hexana senyawa flavonoid terlihat lemah. Kandungan senyawa metabolit sekunder pada ikan buntal Arothron manilensis mengandung komponen senyawa flavonoid. Kandungan senyawa metabolit sekunder flavonoid pada ikan buntal A. manilensis menunjukkan nilai Rf 0.5-0.975 yang memiliki potensi sebagai antioksidan. Diperlukan kembali pengujian High Performance Liquid Chromatography (HPLC), untuk memisahkan molekul senyawa metabolit sekunder dalam waktu minimum.

Kata Kunci: Arothron manilensis, Flavonoid, KLT, Skrining Fitokimia.

ABSTRACT

Secondary metabolites are compounds that are produced by living things to maintain life, as a form of effort to defend themselves from natural predators. Pufferfish are the only marine organisms that have the potential to produce secondary metabolites. The purpose of this research is to analyze the comparisonof eluent solutions and optimal fractions to determine the secondary metabolite compounds of flavonoids in the flesh of the pufferfish Arothron manilensis. The research method was carried out using Phytochemical Screening and Thin Layer Chromatography (TLC) methods to detect secondary metabolites. Research results showed that the determination of the eluent, the polarity of the eluent, and the reagents used greatly affect the appearance of the secondary metabolite compound profile. In the ethyl acetate fraction, the secondary metabolite flavonoid compound is the most prominent, while in the n-hexane fraction, the flavonoid compounds are not visible. The content of secondary metabolites in puffer fish (Arothron manilensis) contains flavonoid compounds. The content of flavonoid secondary metabolites in puffer fish A. manilensis showed an Rf value of 0.5-0.975 which has potential as an antioxidant. High Performance Liquid Chromatography (HPLC) testing is needed again, to separate the secondary metabolite compound molecules in a minimum time.

Keywords: Arothron manilensis, Flavonoids, Phytochemical Screening, TLC


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References


Amano, M., Takatani, T., Sakayauchi, F., Oi, R., and Sakakura, Y. (2022). The Brain of The Wild Toxic Marine Pufferfishes Accumulates Tetrodotoxin. Toxicon, 218, 1-7. https://doi.org/10.1016/j.toxicon.2022.08.015

Arifin, B., dan Ibrahim, S. (2018). Struktur, Bioaktivitas dan Antioksidan Flavonoid. Jurnal Zarah, 6(1), 21-29. https://doi.org/10.31629/zarah.v6i1.313

Dewi, N.W.O.A.C., N.M. Puspawati., I.M.D. Swantara., I.A.R.A. Asih. dan W.S. Rita. (2014). Aktivitas Antioksidan Senyawa Flavonoid Ekstrak Etanol Biji Terong Belanda (Solanum betaceum, syn) dalam Menghambat Reaksi Peroksidasi Lemak pada Plasma Darah Tikus Wistar. Cakra Kimia (Indonesian EJournal of Applied Chemistry), 2(1), 7-16.

El Hady, H. H. A. Daboor, S. M dan Ghoniemy, A. E., Nutritive and Antimicrobial Profiles of Some Seagrasses From Bardawil Lake Egypt. Egyptian Journal of Aquatic Research, 33(3), 103-110.

Harborne, J.B. (1987). Metode Fitokimia Penuntun Cara Modern Menganalisis Tumbuhan. ITB Press. Bandung. 354 hlm.

Intiyani, W.D.M., Yusnaini, dan Sahidin. (2019). Analisis LC-MS/MS (Liquid Crhomatogaph Mass Spectrometry) dan Metabolit Sekunder serta Potensi Antibakteri Ekstrak n-Heksana Spons Callyspongia aerizusa yang Di ambil Pada Kondisi Tutupan Terumbu Karang yang Berbeda Di Perairan Teluk Staring. Jurnal Biologi Tropis, 19(2),134. https://doi.org/10.29303/jbt.v19i2.1126

Misgiati, Winarni, I.,Murniasih, T., Novriyanti, E.,Tarman, K., Safithri, M., Setyaningsih, I., Cahyati, D., Pratama, B.P., and Wirawati, I. (2024). The Anticancer and Antioxidant Potential of Local Sea Cucumber Holothuria Edulis, An Ecology Balancer of Labuan Bajo Marine Ecosystem. Case Studies in Chemical and Environmental Engineering, 9(3), 100625. https://doi.org/10.1016/j.cscee.2024.100625

Sahidin, I.,Sabandar C, W., Wahyuni., Hamsidi R., Malaka H M., Sadarun B., Aslan L O. (2018). A -nor Steroids from the Marine Sponge, Clathria Species. Malaysian Journal of Analytical Sciences, 22(3), 375-382. https://doi.org/10.17576/mjas-2018-2203-02

Saifudin, A. (2014). Senyawa Alam Metabolit Sekunder Teori, Konsep, dan Teknik Pemurnian. Yogyakarta: Deepublish.

Saito, M., Kunisaki, N. (1998). Proximate Composition, Fatty Acid Composition, Free Amino Acid Contents, Mineral Contents, and Hardness of Muscle From Wild and Cultured Puffer Fish Takifugu rubripes. Nippon Suisan Gakkaishi, 64(1), 116-120. https://doi.org/10.2331/suisan.64.116

Septiana, A. T. dan A. Asnani. (2012). Aktivitas Antioksidan Ekstrak Rumput Laut Sargassum duplicatum. Jurnal Teknologi Pertanian, 14(2), 79-86.

Uddin M, Pervin R, Kabir Y, dan Absar N. (2013). Preliminary Screening of Secondary Metabolites and Brine Shrimp Lethality Bioassay of Warm-Water Extracto of Puffer Fish Organs Tissue, Tetraodon cutcutia, Available in Bangladesh. Journal of Biomedical and Pharmaceutical Research. 2(5), 14-18.

White W.T., Last P.R., Dharmadi, Faizah R., Chodrijah U., Prisantoso B.I., Pogonoski J.J., Puckridge M. and Blaber S.J.M. (2013). Market fishes of Indonesia (Jenis-jenis Ikan Di Indonesia). ACIAR Monograph No. 155. Australian Centre for International Agricultural Research:Canberra. 438 pp.

Windono, T., Soediman, S., Yudawati, U., Ermawati, E., Srielita, dan Erowati, T. I. (2001). Uji Peredam Radikal Bebas Terhadap 1,1-Diphenyl-2-Picrylhydrazyl (DPPH) Dari Ekstrak Kulit Buah dan Biji Anggur (Vitis viniferal) Probolinggo Biru dan Bali. Artocarpus, 1(1), 34-43.

Harborne, J. B. (1998). Phytochemical methods: A guide to modern techniques of plant analysis (3rd ed.). Springer.

Sofowora, A. (1993). Medicinal plants and traditional medicine in Africa. Spectrum Books Limited.

Parekh, J., & Chanda, S. (2007). In vitro antimicrobial activity and phytochemical analysis of some Indian medicinal plants. Turkish Journal of Biology, 31(1), 53–58.

Yadav, R. N. S., & Agarwala, M. (2011). Phytochemical analysis of some medicinal plants. Journal of Phytology, 3(12), 10–14.




DOI: https://doi.org/10.21107/juvenil.v6i4.27292

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Juvenil byJurusan Kelautan dan Perikananis licensed under a Creative Commons Attribution 4.0 International License.

Published by: Department of Marine and Fisheries, Trunojoyo University of Madura