ANALISIS BAKTERI PEREDUKSI KONSENTRASI LOGAM TIMBAL Pb(CH3COO)2 MENGGUNAKAN GEN 16S Rrna

Yudi Nurul Ihsan, Kalysta Fellatami, Rega Permana, Yeni Mulyani, Tri Dewi K Pribadi

Abstract


ABSTRACT

This study aims to obtain the type and capabilities of bacteria in the reduction of the concentration of lead metal Pb(CH3COO)2 from sediments of the Karangsong Coast, Indramayu. Methods used namely exploratory methods and the resulting data is analyzed in the descriptive. In molecular identification methods using 16S rRNA Gene which is a universal primer for bacterium. Bacterial sequences obtained from GenBank data analyzed by NCBI sites.The results of this research show that eleven isolates of bacteria that the pore water of sediment derived from the highest reduction of lead metal Pb(CH3COO)2 concentration of 1.5 mgL-1 be 0.364 mgL-1 with a value of efficiency reached 75.7%. Four isolates bacterial with the highest density values were identical to the Albirhodobacter marinus strain N9 (ACC. No. NR 126203.1) with identity value 98% and kinship value 100%, Pseudoalteromonas tetraodonis strain NBRC 103034 (ACC. No. NR 114187.1) with identity value 81% and kinship value 84%, Pseudoalteromonas lipolytica strains LMEB 39 (ACC. No. NR 116629.1) with identity value 98% and kinship value 96%. Pseudoalteromonas lipolytica strains LMEB 39 (ACC. No. NR 116629.1) with identity value 93% and kinship value 93%.

 

Keywords: Bacteria, Bioremediation, 16S rRNA gene, Lead Metal

 

ABSTRAK

Riset ini bertujuan untuk mendapatkan jenis dan kemampuan bakteri dalam mereduksi konsentrasi logam timbal Pb(CH3COO)2 dari sedimen Pantai Karangsong, Indramayu. Metode yang digunakan yaitu metode eksploratif dan data yang dihasilkan dianalisis secara deskriptif.  Metode identifikasi secara molekular menggunakan Gen 16S rRNA yang merupakan primer universal untuk bakteri. Sekuens bakteri yang didapatkan dianalisis dengan data GenBank dari situs NCBI. Hasil riset ini menunjukkan bahwa sebelas isolat bakteri yang berasal dari air pori sedimen paling tinggi mereduksi logam timbal Pb(CH3COO)2 dari konsentrasi 1,5 mgL-1 menjadi 0,364 mgL-1 dengan nilai efisiensi mencapai 75,7%. Empat isolat bakteri dengan nilai kepadatan tertinggi identik dengan Albirhodobacter marinus strain N9 (Acc. No. NR 126203.1) dengan nilai identity 98% dan nilai kekerabatan 100%, Pseudoalteromonas tetraodonis strain NBRC 103034 (Acc. No. NR 114187.1) dengan nilai identitiy 81% dan nilai kekerabatan 84%, Pseudoalteromonas lipolytica strain LMEB 39 (Acc. No. NR 116629.1) dengan nilai identity 98% dan nilai kekerabatan 96%. Pseudoalteromonas lipolytica strain LMEB 39 (Acc. No. NR 116629.1) dengan nilai identity 93% dan nilai kekerabatan 87%.

 

Kata Kunci: Bakteri, Bioremediasi, Gen 16S rRNA, Logam Timbal

 


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DOI: https://doi.org/10.21107/jk.v13i2.7285

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