GHR mRNA Expression in the Liver and Muscles of Local Pigs from The Islands in North Sulawesi, Indonesia

Revolson Alexius Mege, Friska Mery Montolalu, Nonny Manampiring, Debby Jacqueline J Rayer


The GHR gene is known as a member of the growth gene's superfamily that function important in controlling the growth. This study was designed to investigate the expression of GHR genes in the liver and muscles of local pig from the islands in North Sulawesi, Indonesia. The parameters measured was GHR mRNA expression by reverse tranriptase RT-PCR. mRNA sample was taken from liver and longisimus dorsi muscle of sacrifice pigs and cDNA was amplified using specific primer for target genes. The GHR primer gene used was 5’ TTT TCT GGG AGT GAA GCC AC 3' and R = 5’ AGG GCT CTG TAA ACC GTG AC 3' with its housekeeping gene GAPDH. The result showed that GHR is expressed in the liver and muscles of local pig from the islands in North Sulawesi. GHR mRNA expression in muscles was higher than in liver (P>0.05). The GHR gene expression was also regulated by factors such as nutritional intake, GH, ages, temperature and hormones.

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J. Ayuk, and M. C. Sheppard, “Growth hormone and its disorders,” Postgraduate Med. J, vol. 82, pp. 24–30,

Ardiyanti, “Effects of GH gene polymorphism and sex on carcass traits and fatty acid compositions in

Japanese Black cattle,” Anim. Sci. J, vol.80, pp. 62– 69, 2009.

R. M. Akers, “Major advances associated with hormone and growth factor regulation of mammary growth

and lactation in dairy cows,” J Dairy Sci, vol. 89, pp. 1222–1234, 2006.

H. ThidarMyint,” Combined administration of ghrelin and GHRH synergistically stimulates GH release in

Holstein preweaning calves,” Domes Anim. Endocrinol, vol. 34, pp. 118–123, 2008.

S. Djojosoebagio, Fisiologi Kelenjar Endokrin, Penerbit Universitas Indonesia, Jakarta, 1996.

L. Di Stasio, G. Destefanis, A. Brugiapaglia, A. Albera, and A. Rolando, “Polymorphism of the GHR gene in

cattle and relationship with meat production and quality,” Anim. Genet, vol. 36, pp. 138–140, 2005.

L. S. Smit, “The role of the growth hormone (GH) receptor and JAK1 and JAK2 kinases in the activation of

Stats 1, 3, and 5”, Mol. Endocrinol, pp. 10:519–533, 1996.

K. Sjogren , ”Liver-derived insulin-like growth factor I (IGF-I) is the principal source of IGF-I in blood but is

not required for postnatal body growth in mice,” Proc. Natl. Acad. Sci, vol. 96, pp. 7088–7092, 1999.

R. A. Mege, and Y. S. Mokosuli, “DNA Barcoding of local pigs in minahasa, north Sulawesi,” International

Journal of Fauna and Biological Studies, pp. 4(5):82-87, 2017.

D. T. H. Sihombing, Ilmu Peternakan Babi, Gajah Mada University Press (ID), Yogyakarta, 2006.

M. I. Steffl, M. Schweiger, J. Mayer, and W. M. Amselgruber, “Expression and localization of growth

hormone receptor in the oviduct of cyclic and pregnant pigs and mid-implantation conceptuses,”

Histochem. Cell. Biol, vol. 131(6), pp. 773-9, March 2018.

M. Tixier-Boichard, “From phenotype to genotype: Major genes in chickens”,World’s Poultry Sci. J, pp.

:65–75, 2002.

W. Ge, M. E. Davis, H. C. Hines, and K. M. Irvin, “Rapid communication: single nucleotide polymorphisms

detected in exon 10 of the bovine growth hormone receptor gene”, J. Anim. Sci, pp. 78:29–30, 2000.

Sumantri, D. Herdiana, A. Farajallah and D. Rahmat, “Keragaman gen Pituitary-Specific Transcription Factor-

Lokus Pit-1-Hinf1 dan pengaruhnya terhadap bobot tubuh induk, dan produksi susu pada domba lokal”,

JITV, pp. 14: 222-229, 2009.

T. D. Etherton, and D. E. Bauman, “Biology of somatotropin in growth and lactation of domestic animals,”

Physical. Rev, vol. 78, pp. 745-761, 1998.

Sutarno, A. J. Lymbery, R. C. A. Thompson, J. M. Cummins, “Association between growth hormone genotypes and estimated breeding values for pre-weaning growth of beef cattle”, [Proceeding of The

thInternational Congress on Animal Reproduction, P26-19, Sydney June 30 – July 4, 1996].

Bižienė Renata, I. Miceikienė, L. Baltrėnaitė, N. Krasnopiorova, “Association between growth hormone gene polymorphism andeconomic traits in pigs,” Vet. Med. Zoot. Vol. 56, pp. 78, 2011.

L. J. Machlin, ”Effect of porcine growth hormone on growth and carcass composition of the pig,” Journal of

Animal Science, vol. 35 no.4, 1972.

T. D. Schmittgen, and K. J. Livak, “Analyzing real-time PCR data by the comparative CT method,” Nat. Protoc,

vol. 3(6), pp. 1101-1108, 2008.

J. Lin, and C. Redies, “Histological evidence: housekeeping genes beta-actin and GAPDH are of limited value

for normalization of gene expression”, Dev. Genes. Evol, pp. 222:369. 2012.

L. L. Tu, X. L. Zhang, D. Q. Liu, S. X. Jin, J. L. Cao, L. F. Zhu, F. L. Deng, J. F. Tan, and C. B. Zhang, “Suitable

internal control genes for qRT-PCR normalization in cotton fiber development and somatic embryo

genesis,” Chin. Sci. Bull, vol. 52, pp. 3110-3117, 2007.

M. F. Rothschild, Zhi-liang Hu, and Zhihua Jiang, “Advances in QTL Mapping in Pigs,” J. Biol. Sci, vol. 3(3), pp.

–197, 2007.

M. Murphy, K. G. Meade, P. A.Hayes, S. D. Park, A. C. Evans, P. Lonergan, D. E. Machugh, “Transmission ratio distortion at the growth hormone gene (GH1) in bovine preimplantation embryos: An in vitro culture- induced phenomenon?,” Mol. Reprod. Dev, pp. 75: 715-722, 2008.

M. J. Dauncey, K. A. Burton, P. White, A. P. Harrison, R. S. Gilmour, C Duchamp and D. Cattaneo, “Nutritional regulation of growth hormone receptor gene expression,” FASEB J, pp. 8:81–88, 1994.

M. Katsumata, D. Cattaneo, D. White, P. Burton, and M. J. Dauncey, “Growth hormone receptor gene expression in porcine skeletal and cardiac muscles is selectively regulated by postnatal undernutrition,” J. Nutr, vol. 130, pp. 2482–2488, 2000.

G. A. Aguirre, D. I. J. Rodriguez, R. G. Garza, and C. I. Cortazar, “Insulin-like growth factor-1 deficiency and

metabolic syndrome,” J. Transl. Med, pp. 14:3, 2006.


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