Beneficial of Clover Leaf (Marsilea crenata) as Bio-adsorbent of Heavy Metal Contamination in Red Mussels (Masculita senhausia)

S Winarti1, E K B Susiloningsih, A Lee


Clover (Marsilea crenata) is one type shrub plant that is widely used as a vegetable in Surabaya, Indonesia, rich in phytochemical compounds. One of them is polyphenols which have the ability to chelate metals. Chaempherol, quercetine, routine, flavonols are types of polyphenols that can be used as chelating agents for heavy metals because they have functional groups to bind metals. The objective of the research was to study the effect of clover leaf flour concentration and contact time on decreasing heavy metal contamination in red mussels. This study used a factorial completely randomized design with two replications. The first factor were the concentration of clover leaf flour (1%, 3%, 5%) and factor II were the contact time (0 minutes as a control, 60 minutes, 90 minutes, 120 minutes). The data from this research analysis using ANOVA (analysis of variant) and DMRT (duncan't multiple range teste). The best treatment was concentration of 5% clover leaf flour and contact time 120 minutes can reduce copper from 47.49 ppm to 19.69 ppm (decrease 58.54%), lead from 21.31 ppm to 9, 67 (decrease 54.67%), mercury from 0.54 ppm to 0.26 ppm (decrease 52.32%) in red mussels

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