The Influence of purple sweet potato toward Fas Epitel of White Rat

M E Fitriasnani, A Nikmatul, G P Yanuaringsih, S Aminah


This study builds on proving the purple sweet potato anthocyanin can decrease the epitel Fas cell of ovariectomy of white rat expression. The research design used was true experimental with  Randomized post only control group design. The trial animal was female white rat (Rattus norvegicus) aged 9-12 weeks, weight 150-250 gram namely 30 divided into 5 groups: negative control group, positive control group and t giving group with the dose 20 mg/kg weight (A1), dose 40 mg/kg weight (A2), dose 80 mg/kg weight (A3). It is done by ovariectomy during 28 post ovariectomy then giving purple sweet potato anthocyanin during 30 days. The expression of Fas was examined by using IHC. To increase the reliability of the data was analyzed with Independent Sample T Test dan One Way Annova. The result shows that the purple sweet potato anthocyanin can decrease he Fas expression to endometrium cell epitel with the dose 40 mg/kg weight and 80 mg/kg weight significantly. It is caused the purple sweet potato anthocyanin can decrease dead cell of extrinsic. The dead cell was passed by the extrinsic ways. The finding has implication for can decrease the Fas expression of ovariectomy female white rat.

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Burger HG, Hale GE, Robertson DM, Dennerstein L. A review of hormonal changes during the menopausal transition : focus on findings from the Melbourne Women ’ s Midlife Health Project. Hum Reprod Update. 2007;13(6):559–65.

Mendoza-nu M, Rosado-pe J, Santiago-osorio E. Aging Linked to Type 2 Diabetes Increases Oxidative

Stress and Chronic Inflammation. Rejuvenation Res. 2011;14(1):25–31.

Agarwal A, Gupta S, Sharma RK. Role of oxidative stress in female reproduction. Biomed Cent.


Gamal D, Elkholi E. Unexplained postmenopausal uterine bleeding from atrophic endometrium :

Histopathological and hormonal studies. Middle East Fertil Soc J [Internet]. Middle East Fertility Society;

;20(4):262–70. Available from:

Doshi SB, Agarwal A. The role of oxidative stress in menopause. J Midlife Health. 2013;4(3):140–7.

Mcilwain DR, Berger T, Mak TW, Silke J, Meier P, Chan FK. Caspase Functions in Cell Death and

Disease. Cold Spring Harb Perspect Biol. 2014;1–28.

Sato T, Fukazawa Y, Kojima H, Ohta Y, Iguchi T. Multiple mechanisms are involved in apoptotic cell death in the mouse uterus and vagina after ovariectomy. Reprod Toxicol. 2003;17:289–97.

Mor G, Straszewski S, Kamsteeg M. The Fas / Fas Ligand System in Reproduction : Survival and

Apoptosis. Sci World J. 2002;2:1828–42.

Song J, Rutherford T, Naftolin F, Brown S, Mor G. Hormonal regulation of apoptosis and the Fas and Fas ligand system in human endometrial cells. Mol Hum Reprod. 2002;8(5):447–55.

Suhardi C J, Ratnawati R KH. KULTIVAR GUNUNG KAWI DALAM MENINGKATKAN KADAR SUPEROXIDE DISMUTASE PADA TIKUS ( Rattus norvegicus ) DENGAN DIET ATEROGENIK Christian Julio Suhardi *, Retty Ratnawati ** , Husnul Khotimah *** Abstrak EFFECT OF ANTHOCYANIN FROM PURPLE SWEET POTATO ( Ipomo. Maj Kesehat FKUB. 2016;3(4):166–73.

Wang L, Stoner GD. Anthocyanins and their role in cancer prevention. Cancer Lett. 2008;269:281–90. [12] Ano MK, Akayanagi TT, Arada KH, Akino KM, Shikawa FI. Antioxidative Activity of Anthocyanins

from Purple Sweet Potato , Ipomoera batatas Cultivar Ayamurasaki. BioschiBiotechnol,Biochem.


Ruder EH, Hartman TJ, Blumberg J, Goldman MB. Oxidative stress and antioxidants : exposure and

impact on female fertility. Hum Reprod Update. 2008;14(4):345–57.

Sa MA, Arronte-rosales A, Correa-mun E. Menopause as risk factor for oxidative stress. Menopause J North Am Menopause Soc. 2012;19(3):361–7.

Wang Z, Chandrasena ER, Yuan Y, Peng K, Breemen RB Van, Thatcher GRJ, et al. Redox Cycling of Catechol Estrogens Generating Apurinic / Apyrimidinic Sites and 8-oxo-Deoxyguanosine via Reactive Oxygen Species Differentiates Equine and Human Estrogens. ChemResToxicol. 2010;23(8):1365–73.

Hongmei Z, Vogt C. Extrinsic and Intrinsic Apoptosis Signal Pathway Review Scientist. Intech.

;Chapter 1:3–22.

Slayden OVD, Rubin JS, Lacey DL, Brenner RM, Sciences R, Regional O. Effects of Keratinocyte Growth Factor in the Endometrium of Rhesus Macaques during the Luteal- Follicular Transition *. J Endocrinol Metab. 2015;85(1):275–85.

Susan elmore. Apoptosis : A Review of Programmed Cell Death. Toxicol phatology. 2007;35:495–516. [19] Schmitt E, Stopper H, Schmitt E, Stopper H. Estrogenic Activity of Naturally Occurring Anthocyanidins

Estrogenic Activity of Naturally Occurring Anthocyanidins. Nutr Cancer. 2011;41(1 & 2):37–41. [20] Mazza GJ. Anthocyanins and heart health. Pacific Agri-Food Res Cent. 2007;43(Figure 1):369–74.

Ruong VANENT, Eighton NID, Hompson ROTT, Eeters ROFMCF, Ean LISAOD, Ecota KEVP, et al.

Characterization of Anthocyanins and Anthocyanidins in Purple-Fleshed Sweetpotatoes by HPLC-DAD / ESI-MS / MS. Agric Food Chem. 2010;58(1):404–10.

Mohanraj R S. Sweet Potato ( Ipomoea batatas [ L .] Lam ) - A Valuable Medicinal Food : A Review. J

Med Food. 2015;7(February):733–41.

Ishida H, Suzuno H, Sugiyama N, Innami S. Nutritive evaluation on chemical components of leaves , stalks and stems of sweet potatoes ( Ipomoea batatas poir ). Food Chem. 2000;68:359–67.

Stegh AH, Schickling O, Ehret A, Scaffidi C, Hofmann TG, Grummt I, et al. DEDD , a novel death effector domain-containing protein , targeted to the nucleolus. Embo J. 1998;17(20):5974–86.

Scaffidi C, Kirchhofft S, Krammert PH, Peter ME. Apoptosis signaling in lymphocytes Carsten Scaffidi*, Sabine Kirchhofft, Peter H Krammert and Marcus E Peter: Immunology. 1999;11:277–85.

Sedar G, Njateng S, Du Z, Gatsing D, Mouokeu RS, Liu Y. Antibacterial and antioxidant properties of crude extract , fractions and compounds from the stem bark of Polyscias fulva Hiern ( Araliaceae ). BMC

Complement Altern Med. BMC Complementary and Alternative Medicine; 2017;17:1–8.


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