VARIAN GENETIK Sardinella lemuru DI PERAIRAN SELAT BALI

Gde Raka Angga Kartika, Aida Sartimbul, W Widodo

Abstract


Sardinella lemuru merupakan salah satu spesies ikan yang mendominasi di perairan Selat Bali. Identifikasi Sardinella lemuru dengan sardinella lain di perairan Selat Bali hanya dibedakan berdasarkan secara morfologi, sedangkan identifikasi genetik belum pernah dilakukan dan variasi gentetik juga belum diketahui. Penelitian ini bertujuan memastikan jenis Sardinella lemuru di perairan Selat Bali dan mengetahui variasi genetik dan kekerabatannya berdasarkan  gen Cythocrome oxidase sub unit 1 (COI). Metode yang dilakukan adalah dengan mengisolasi DNA dari organ tubuh ikan lemuru dan hasilnya diamplifikasi dengan gen COI, kemudian dilakukan skuensing untuk mendapatkan sekuen data ikan  lemuru perairan Selat Bali dan urutan basa hasil skuensing dianalisis menggunakan program MEGA 5.2. Hasil menunjukan bahwa ikan lemuru di Selat Bali termasuk kedalam spesies Sardinella lemuru dengan tingkat kesamaan analisis BLAST adalah sebesar 98-100%. 11 sampel skuen ikan lemuru membentuk 2 kelompok besar. Hasil penelitian juga menunjukkan bahwa spesies Sardinella lemuru dan Sardinella longiceps merupakan speies ikan yang berbeda dengan jarak genetik >0,019

Kata Kunci: COI, Sardinella lemuru, Selat Bali

GENETIC VARIANCE OF Sardinela lemuru IN THE BALI STRAIT WATERS

Sardinella lemuru is one of the fish species that dominate in the Bali Strait. identification Sardinella lemuru with the others Sardinella in the Strait of Bali is based on morphological characteristics, whereas genetic identification of Sardinella lemuru in bali strait has not been done and variations genetic also unknown. This research aims to ascertain the type of sardinella lemuru in the bali strait and Determine genetic variation and kinship based on cytochrome oxidase subunit  (COI)  gene. The method is performed by isolating DNA from fish organs and the results are amplified by the COI gene skuensing then performed to obtain the data sequence of Sardinella lemuru Bali Strait and analyzed using the program MEGA 5.2. Results showed that lemuru in Bali Strait is sardinella lemuru species with the degree of similarity BLAST analysis of 98-100%. 11 samples skuen lemuru forming two large clad. The results also showed that the species Sardinella lemuru and Sardinella longiceps  different speies with genetic distance> 0,019.

Keywords: COI, Sardinella lemuru, Bali Straits


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References


Ausubell, F. M, Brent, R., Kingston, R. E., Moore, P.D., Seidemen, J. G., Smith, J. A., & Struhl, K. (2002). Short Protocols in Molecular Biology, Fifth Edition. John Willey and Son Inc. Canada

Avise, J. C. (1994). Molecular Marker, Natural History and Evolution. Chapman and Hall. USA

Buhay, E. (2009). “COI-Like” Sequences Are Becoming Problematic In Molecular Systematic And DNA Barcoding Studies. University Of South Carolina

Costa, F. O., de Waard, J. R., Boutillier, J., Ratnasingham, S., Dooh, R. T., Hajibabei, M., & Hebert. P. D. N. (2007). Biological Identifications through DNA Barcodes: The Case of Crustacea. Canadian J. Fish. & Aqua. Sci., 64(2), 272-295. doi: 10.1139/f07-008

Divya, P., Gopalakrisna, A., Jhon, L., Thomas, P.C., & Cakra, W. S. (2009). Mithocondrial DNA (Cythocrome C Oxidase I) Sequencing of Indian Marine Mussels. Indian J. Fish., 56(3), 223-226.

Dunham, R. A. (2002). Aquaculture and Fisheries Biotechnology: Genetic Approach. New York: CABI Publishing, Cambridge. pp 85-99

Gordon, L. (2005). Oceanography of the Indonesian Seas and Their Through flow. Oceanography, 18(4).

Hebert, P. D. N., Cywinska, A., Shelley, L., & de Waard, J. R. (2003). Biological Identifications Through DNA Barcodes. Proc. R. Soc. Lond., 270.

Hebert, P. D. N., Ratnasingham, S., & de Ward, J. R. (2003). Barcoding Animal Life Cytochrome C Oxidase Divergences Among Closely Related Species. Proc. R. Soc. Lond., 270.

Jonas, P. Q., Brian, S., Santos, P., Ong., Zubaida, U., Basiao., Ian Kendrich, C., Fontanilla, & Cao, E. P. (2011). DNA Barcoding of the Philippine Endemic Freshwater Sardine Sardinella tawilis (Clupeiformes: Clupeidae) and Its Marine Relatives. Philipp. Agric. Scientist, 94(3), 248-257.

Liu, W., Lathrop, A., Fu, J., Yang, D., & Murphy, R. W. (2000). Phylogeny of East Asian Bufonids Inferred from Mitochondrial DNA Sequences (Anura: Amphibia). Molecular Phylogenetics and Evolution, 14(3), 423-435.

Nei, M., & Kumar, S. (2000). Molecular Evolution and Phylogenetics. Oxford University Press, New York.

Noor, R. (2008). Genetika Ekologi. Laboratorium Genetika Ternak. Institut Pertanian Bogor

Rizal, F., Setiyo, W., Pratomo, Y., dan Andri, E. (2015). Identifikasi Komponen Harmonik Di Selat Lombok Berdasarkan Data Arus Time Series. Depik, 4(1), 24-32.

Sofro, A. S. M. (1994). Keanekaragaman Genetik. Yogyakarta: Andi Offset. 127 hal

Susilo, K. (2015). Variabilitas Faktor Lingkungan Pada Habitat Ikan Lemuru Di Selat Bali Menggunakan Data Satelit Oseanografi Dan Pengukuran Insitu.

Tjong, D. H., Matseu, M., Kuramoto, M., Belabut, D. M., Sen, Y. H., Nishioka, M., & Sumida, M. (2007). Morphological Divergence, Reproductive Isolating Mechanism and Molecular Phylogenetic Relationship, Among Indonesia, Malaysia, and Japan Populations of the Fejervaria limnocharis Complex (Anura, Ranidae). Zoological Science, 24, 1197- 1212.

Tubaro, P., & Lijtmaer, D. A. (2002). Hybridization Patterns and The Evolution of Reproductive Isolation in Ducks. División Ornitología, Museo Argentino de Ciencias Naturales ‘Bernardino Rivadavia’ Buenos Aires, Argentina. Biological Journal of the Linnean Society, 197-200

Tomascik, T., Mah, A. J., Nontji, A., Moosa, M. K. (1997). The ecology of Indonesian seas. Part I, Periplus Editions Ltd., Singapore

Wijana, I. M. S., & Mahardika, I. G. N. (2010). Struktur Genetik Dan Filogeni Tuna Sirip Kuning (Thunnus albacares) Berdasarkan Sekuen DNA Mitkondria Control Region Sitokrom Oksidase I Pada Diversitas Zone Biogeografi. Jurnal Bumi Lestari, 10(2): 270 – 274.

Zhang, J. (2011). Species Identification of Marine Fishes in China with DNA Barcoding. Evidence-Based Complementary and Alternative. Medicine, Article ID 978253, 10 pages.




DOI: http://dx.doi.org/10.21107/jk.v10i1.1615

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ISSN: 1907-9931 (Print), 2476-9991 (Online)